User:Trisha I. Ibeh/Notebook/Trisha Notebook/2014/04/15: Difference between revisions

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#Add 1.3 mL of deionized water to sample
#Add 1.3 mL of deionized water to sample
#Remove deionzed water from sample
#Remove deionzed water from sample
#Repeat 3 times
#Repeat steps 3 and 4 three times
#Place sample over UV light (fluorescence should be seen within the hydrogels)
#Place sample over UV light (fluorescence should be seen within the hydrogels)
#Vortex sample for 2 minutes
#Vortex sample for 2 minutes

Revision as of 01:47, 7 May 2014

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Objective

  1. Test fluorescence of nanoparticles
  2. Microscope Analysis
  3. Preparation of sonicated samples

Microscope Analysis

Please refer to Puja Mody's Open Wetware Notebook for microscope analysis of samples

Sample Prep for Stock Solutions

Table 1: BSA Stock Prep

Table 2: FeSO4 Stock Prep

Table 3: Hemoglobin Prep

Table 4: KNO3 Prep

Table 5: KOH Prep

Table 6: Gold Prep

Table 7: Magnetite/ BSA Prep

Table 8: Magnetite/ Hemoglobin Prep

Table 9: Gold added to Magnetite/ BSA Prep

Table 10: Gold added to Magnetite/ Hemoglobin Prep

Atomic Absorption

Table 1: Preparation of iron standard used fro AA

Fluorescence Testing

Procedure

  1. Centrifuge 1.5 eppendorf tube with dye samples for 15 minutes
  2. Use micropipette to remove supernatant
  3. Add 1.3 mL of deionized water to sample
  4. Remove deionzed water from sample
  5. Repeat steps 3 and 4 three times
  6. Place sample over UV light (fluorescence should be seen within the hydrogels)
  7. Vortex sample for 2 minutes
  8. Apply magnetic field to sample
  9. Repeat steps 7 and 8 continuously for approximately 4 minutes
  10. Test the deionized water for fluorescence


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