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*[[OpenWetWare:Welcome|Introductory tutorial]] | *[[OpenWetWare:Welcome|Introductory tutorial]] | ||
*[[Help|OpenWetWare help pages]] | *[[Help|OpenWetWare help pages]] | ||
==Module 2 Datums== | |||
Primer Sequences: | |||
Forward Primer: | |||
5’ – tgt tgt cta tgt tta att aac cta tgt tgt gac gtt ttt TCC ATG GAA AAG AGA AGA TG – 3’ | |||
(where lowercase codons are from the flap sequence, and uppercase codons are in the landing sequence for the TAP-TRP fusion) | |||
Tm = 64.5°C | |||
GC content = 32.2% | |||
Reverse primer: | |||
5’ – gat gtt ctt tcc ata cta att ttt agc tgt cga tta gaa TAC GAC TCA CTA TAG GGC GA – 3’ | |||
(where lowercase codons are from the flap sequence, and uppercase codons are in the landing sequence for the TAP-TRP fusion) | |||
Tm = 65.2°C | |||
GC content = 37.3% |
Revision as of 12:14, 7 October 2008
I am a new member of OpenWetWare!
Contact Info
- Troy T Rurak
- MIT
- Simmons Hall, Rm665, 229 Vassar St., Cambridge, MA 02139
- trurak AT mit DOT edu
Hey Everyone....I'm a junior in Course 20 (thinking about doubling in 7). After my time here at MIT, I hope to pursue a graduate degree in Toxicology or BME or sometime similar, then move on to med school to later specialize in Anesthesiology. Shoot me an email or
Education
- Year, PhD, Institute
- Year, MS, Institute
- Year, BS, Institute
Research interests
- Interest 1
- Interest 2
- Interest 3
Publications
Useful links
Module 2 Datums
Primer Sequences: Forward Primer: 5’ – tgt tgt cta tgt tta att aac cta tgt tgt gac gtt ttt TCC ATG GAA AAG AGA AGA TG – 3’ (where lowercase codons are from the flap sequence, and uppercase codons are in the landing sequence for the TAP-TRP fusion) Tm = 64.5°C GC content = 32.2%
Reverse primer: 5’ – gat gtt ctt tcc ata cta att ttt agc tgt cga tta gaa TAC GAC TCA CTA TAG GGC GA – 3’ (where lowercase codons are from the flap sequence, and uppercase codons are in the landing sequence for the TAP-TRP fusion) Tm = 65.2°C GC content = 37.3%