User:Troy T Rurak: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
Line 29: Line 29:
*[[OpenWetWare:Welcome|Introductory tutorial]]
*[[OpenWetWare:Welcome|Introductory tutorial]]
*[[Help|OpenWetWare help pages]]
*[[Help|OpenWetWare help pages]]
==Module 2 Datums==
Primer Sequences:
Forward Primer:
5’ – tgt tgt cta tgt tta att aac cta tgt tgt gac gtt ttt TCC ATG GAA AAG AGA AGA TG – 3’
(where lowercase codons are from the flap sequence, and uppercase codons are in the landing sequence for the TAP-TRP fusion)
Tm = 64.5°C
GC content = 32.2%
Reverse primer:
5’ – gat gtt ctt tcc ata cta att ttt agc tgt cga tta gaa TAC GAC TCA CTA TAG GGC GA – 3’
(where lowercase codons are from the flap sequence, and uppercase codons are in the landing sequence for the TAP-TRP fusion)
Tm = 65.2°C
GC content = 37.3%

Revision as of 12:14, 7 October 2008

I am a new member of OpenWetWare!

Contact Info

Troy T Rurak (an artistic interpretation)

Hey Everyone....I'm a junior in Course 20 (thinking about doubling in 7). After my time here at MIT, I hope to pursue a graduate degree in Toxicology or BME or sometime similar, then move on to med school to later specialize in Anesthesiology. Shoot me an email or

Education

  • Year, PhD, Institute
  • Year, MS, Institute
  • Year, BS, Institute

Research interests

  1. Interest 1
  2. Interest 2
  3. Interest 3

Publications

Useful links

Module 2 Datums

Primer Sequences: Forward Primer: 5’ – tgt tgt cta tgt tta att aac cta tgt tgt gac gtt ttt TCC ATG GAA AAG AGA AGA TG – 3’ (where lowercase codons are from the flap sequence, and uppercase codons are in the landing sequence for the TAP-TRP fusion) Tm = 64.5°C GC content = 32.2%

Reverse primer: 5’ – gat gtt ctt tcc ata cta att ttt agc tgt cga tta gaa TAC GAC TCA CTA TAG GGC GA – 3’ (where lowercase codons are from the flap sequence, and uppercase codons are in the landing sequence for the TAP-TRP fusion) Tm = 65.2°C GC content = 37.3%