|
>Rename user script |
| (16 intermediate revisions by 2 users not shown) |
| Line 1: |
Line 1: |
| | | #REDIRECT [[Ty Thomson]] |
| == Thesis Topic ==
| |
| | |
| The main objectives of my work is to develop the tools to perform time-dependent stimulation and analysis of signaling pathways, and show that this is more powerful than traditional time-independent or step response analysis. I am using a computational model of the prototype system, the yeast pheromone response pathway, to generate hypotheses about the pathway. In order to test these hypotheses, time-dependent stimuli will be delivered to cells via a microfluidic device, and in vivo fluorescent reporters will be used to observe the system state. In addition to showing the strengths of this new approach to studying biological systems, I would like to use it to further our understanding of the pheromone response pathway.
| |
| | |
| == Research Goals ==
| |
| | |
| My research can be broken down into 4 main goals (that follow for the most part chronologically).
| |
| | |
| #Build a model of the pheromone response pathway | |
| #*Develop a model of the pheromone response pathway that can be used in conjunction with time-dependent stimulation and analysis of the pathway to propose and test hypotheses. Once completed, this model can be used as a predictive tool for pathway response.
| |
| #Build a microfluidic device for time-dependent stimulation of cells
| |
| #*Design, build and characterize a device to allow for rapid variation of extracellular conditions for cells fixed in a microfluidic channel.
| |
| #Investigate the pathway with time-dependent stimulation
| |
| #*Examine the frequency filtering characteristics of the pheromone response pathway in order to study the limits of propagation of time-varying signals through the pathway. Use the model to form and test hypotheses generated by studying the response of the pathway to time-dependent stimulation.
| |
| #Identify and apply techniques for non-linear system identification
| |
| #*Identify and apply tools developed for other fields to the analysis of signaling pathways, particularly with respect to time-dependent stimulation.
| |
