User:Victor Tapia: Difference between revisions
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<!-- Feel free to add brief descriptions to your research interests as well --> | <!-- Feel free to add brief descriptions to your research interests as well --> | ||
# Modularity of Protein Structure and Cellular Signal Transduction. | # Modularity of Protein Structure and Cellular Signal Transduction. | ||
# Protein Folding and | # Protein Folding and Neurodegenerative Deseases. | ||
# Natural Autoimmunology and Diagnostic Assays | # Natural Autoimmunology and Diagnostic Assays | ||
Revision as of 00:43, 3 February 2009
I am a new member of OpenWetWare!
Contact Info
- Victor Tapia
- Charité - Medical School
- hessischer Str. 3-4
- 10115 Berlin
- Berlin, Germany
- Email me through OpenWetWare
I work in the Inst. f. Med. Immunol. at the Charité Medical School of Berlin. I learned about OpenWetWare from friends, and I've joined for knowledge interchange.
Education
- 2009?, PhD, Institute for Medical Immunology, Charité Medical School, Berlin
- 2004, MS, Institute of Biology, Humboldt University, Berlin
- 2002, BS, Institute of Biology, Humboldt University, Berlin
Research interests
- Modularity of Protein Structure and Cellular Signal Transduction.
- Protein Folding and Neurodegenerative Deseases.
- Natural Autoimmunology and Diagnostic Assays
Publications
-
Tapia & Volkmer (2009) "Exploring and profiling protein function with peptide arrays" in: Methods in molecular biology (eds. Chiari). Humana Press
- Tapia V, Ay B, Triebus J, Wolter E, Boisguerin P, and Volkmer R. Evaluating the coupling efficiency of phosphorylated amino acids for SPOT synthesis. J Pept Sci. 2008 Dec;14(12):1309-14. DOI:10.1002/psc.1068 |
This work reviews the peptide array technology focusing on technological advancements, oriented immobilization of peptide probes, and some interesting applications. Going along the philosophy of the monography series, we give some general recomendations on methods and instruments.
- Tapia V, Bongartz J, Schutkowski M, Bruni N, Weiser A, Ay B, Volkmer R, and Or-Guil M. Affinity profiling using the peptide microarray technology: a case study. Anal Biochem. 2007 Apr 1;363(1):108-18. DOI:10.1016/j.ab.2006.12.043 |
This work analyses the predictive potential of microarray-based binding assays. Fluorescent SI-measurements were used to fit a mass-action derived model estimating the observed affinity (equil. diss. const.).
- Weiser AA, Or-Guil M, Tapia V, Leichsenring A, Schuchhardt J, Frömmel C, and Volkmer-Engert R. SPOT synthesis: reliability of array-based measurement of peptide binding affinity. Anal Biochem. 2005 Jul 15;342(2):300-11. DOI:10.1016/j.ab.2005.04.033 |
This work analyses the quantitative potential of measurements derived from peptide macroarray-based immunobinding assays. Chemoluminiscent signal intensity data was confronted with independent "golden stardard" measurements (surface plasmon resonance-based binding assays on a BIAcoreX maschine).
