User:Vijay/Protocol/microarray: Difference between revisions
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< User:Vijay | Protocol
(New page: ==Overview== ==Materials== *Perfect Western (GenHunter Corp) ==Procedure== #SDS-PAGE and TRANSFER done as per our lab protocol ==Buffers== ==1M KH2P04== (No autoclave, no filter, j...) |
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Revision as of 09:59, 5 September 2007
Overview
Materials
- Perfect Western (GenHunter Corp)
Procedure
- SDS-PAGE and TRANSFER done as per our lab protocol
Buffers
1M KH2P04
(No autoclave, no filter, just make it and use it.... in 100ml glass bottle) 13.6gms in 100ml DEPC water
1M K2HP04
(No autoclave, no filter, just make it and use it... in 100ml glass bottle) 22.8gms in 100ml DEPC water
Phosphate Buffer (1 M KP04)
(no autoclave, no filter...just make it and use it in a 50ml falcon tube) 0.5ml 1M KH2P04 9.5ml 1M K2HP04
10ml
Phosphate WASH Buffer (Cloudy)
(no autoclave, no filter...just make it and use it in a 50ml falcon tube) 0.25ml 1M KP04 7.625ml DEPC treated water 42.125ml 95% Ethanol (2.5ml DEPC water + 47.5ml 100% ethanol)
50ml
Phosphate Elution Buffer (4mM)
(no autoclave, no filter... just make it and use it in a 50ml falcon tube) 200ul 1M KP04 to 50ml using DEPC water
