User:Vijay/Protocol/urease-assay

Overview

Urease agar assay is used to differentiate micro organisms based on their urease producing ability.

Materials

• BBL Urea agar base concentration 10X (Becton, Dickinson and Company (BD), Sparks, MD)
• Granulated agar

Procedure

1. Dissolve 1.7g of granulated agar in 100 ml of dH20.
2. Boil it for 40sec in a microwave
3. Autoclave the boiled agar at 121C, 15min, 15lb
4. Cool the agar to 50C
5. Add 10ml room temperature Urea Agar Concentrate-10X to the agar
6. Pour in to plates (5 plates)
7. Streak wild type, mutant on to the plate
8. Incubate wild type, mutant and a control at 37C
9. Watch for change in the color from yellow to pink
10. A pink color indicates presence of Urease activity