User:Vincent Rouilly/Hands On BioBrick Activity: Difference between revisions
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==Overview== | ==Overview== | ||
{| border="1" cellpadding="5" cellspacing="0" align=" | {| border="1" cellpadding="5" cellspacing="0" align="center" style="width:100%" | ||
|- | |- | ||
| colspan="1" style="background:#efefef;" | '''Overview''' | | colspan="1" style="background:#efefef;" | '''Overview''' | ||
| colspan="1" style="background:#efefef;" | '''General setting''' | | colspan="1" style="background:#efefef;" | '''General setting''' | ||
|- | |- | ||
| [[Image:HandsOnBioBrick_ActivityTable.png| | | [[Image:HandsOnBioBrick_ActivityTable.png|400px|Hands on BioBrick table]] | ||
| [[Image:HandsOnBioBrick_IO_Modules.png| | | [[Image:HandsOnBioBrick_IO_Modules.png|400px|Hands on BioBrick IO modules]] | ||
|- | |- | ||
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** a set of available biobricks implemented as Lego bricks filled with some opto-electronic components. | ** a set of available biobricks implemented as Lego bricks filled with some opto-electronic components. | ||
** a conductive surface | ** a conductive surface | ||
** a set of fiber optics to | ** a set of fiber optics to link the different genetic circuits. | ||
| | | | ||
* the activity offers a set of possible inputs for the genetic circuits to be built: | * the activity offers a set of possible inputs for the genetic circuits to be built: | ||
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==Components and Schematics== | ==Components and Schematics== | ||
{| border="1" cellpadding="5" cellspacing="0" align="center" style="width:100%" | |||
|- | |||
| colspan="1" style="background:#efefef;" | '''Constitutive Promoter''' | |||
| colspan="1" style="background:#efefef;" | '''Activated Promoter''' | |||
| colspan="1" style="background:#efefef;" | '''Repressed Promoter''' | |||
|- | |||
| [[Image:HandsOnBioBrick_ConstitutivePromoter.png|400px|Constitutive Promoter Component]] | |||
| [[Image:HandsOnBioBrick_ActivatedPromoter.png|400px|Activated Promoter Component]] | |||
| [[Image:HandsOnBioBrick_RepressedPromoter.png|400px|Repressed Promoter Component]] | |||
|- | |||
| | |||
* Strength of the promoter is set through the potentiometer | |||
* A battery is embedded into the lego brick | |||
* The Lego brick is sealed with a conductive plate to make contact with the table. | |||
| | |||
* Activity of the promoter is driven by the amount of light received on the photo-sensor. | |||
* A battery is embedded into the lego brick | |||
* The Lego brick is sealed with a conductive plate to make contact with the table. | |||
| | |||
* Activity of the promoter is driven by the amount of light received on the photo-sensor. | |||
* A battery is embedded into the lego brick | |||
* The Lego brick is sealed with a conductive plate to make contact with the table. | |||
|- | |||
| colspan="1" style="background:#efefef;" | '''RBS''' | |||
| colspan="1" style="background:#efefef;" | '''Protein Coding Region''' | |||
| colspan="1" style="background:#efefef;" | '''Stop Codon''' | |||
|- | |||
| [[Image:HandsOnBioBrick_RBS.png|400px|Constitutive Promoter Component]] | |||
| [[Image:HandsOnBioBrick_ProteinCodingRegion.png|400px|Activated Promoter Component]] | |||
| [[Image:HandsOnBioBrick_StopCodon.png|400px|Repressed Promoter Component]] | |||
|- | |||
| | |||
* Strength of the RBS is set through the potentiometer | |||
| | |||
* a 10-LEDs graph bar is used to indicate the level of expression. | |||
* a LED is used separately to potentially drive another promoter through a fiber optic. | |||
| | |||
* The stop codon enables the circuit to connect to earth through the table | |||
|} |
Revision as of 11:19, 6 June 2007
Vincent 13:42, 6 June 2007 (EDT): feel free to comment on the content of the activity described and its design.
Context
- Following last year | Imperial College participation to iGEM, my group is putting together an exhibit for the Royal Society Summer Science Exhibition 2007.
- We feel it is a great opportunity to engage with the Public on the concepts behind Synthetic Biology.
- The exhibit attracts a diverse audience from school kids to science enthusiasts.
- I have started to design a hands-on activity, and describe its early design below.
Intentions behind the activity
- To set the background in terms of Molecular and Celluar Biology:
- DNA, Central Dogma, Cells and Bacteria
- Genetic Engineering techniques
- To touch on Synthetic Biology concepts:
- BioBricks
- Modularity
- Re-usability
- To give an opportunity to build simple genetic circuits based on opto-electronic components:
- Protein Generator
- Inverter, Toggle switch & Repressilator
- Link circuits with inputs such as: temperature sensor, Infra-red sensor, Touch sensor, Humidity sensor.
- Link circuits with outputs such as: flagella motor, secretion fan.
Overview