User:Vincent Rouilly/Hands On BioBrick Activity: Difference between revisions
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| [[Image:HandsOnBioBrick_ProteinGenerator.png|300px|Constitutive Promoter Component]] | | [[Image:HandsOnBioBrick_ProteinGenerator.png|300px|Constitutive Promoter Component]] | ||
| [[Image:HandsOnBioBrick_FusionProteinGenerator.png|300px|Activated Promoter Component]] | | [[Image:HandsOnBioBrick_FusionProteinGenerator.png|300px|Activated Promoter Component]] | ||
| [[Image: | | [[Image:HandsOnBioBrick_MonocystronicProteinGenerator.png|300px|Repressed Promoter Component]] | ||
|} | |||
==Elementary Genetic Circuits== | |||
{| border="1" cellpadding="5" cellspacing="0" align="center" style="width:100%" | |||
|- | |||
| colspan="1" style="background:#efefef;" | '''Inverter''' | |||
| colspan="1" style="background:#efefef;" | '''Repressillator''' | |||
|- | |||
| [[Image:HandsOnBioBrick_Inverter.png|300px|Constitutive Promoter Component]] | |||
| [[Image:HandsOnBioBrick_Repressilator.png|300px|Activated Promoter Component]] | |||
|} | |} |
Revision as of 11:35, 6 June 2007
Vincent 13:42, 6 June 2007 (EDT): feel free to comment on the content of the activity described and its design.
Context
- Following last year | Imperial College participation to iGEM, my group is putting together an exhibit for the Royal Society Summer Science Exhibition 2007.
- We feel it is a great opportunity to engage with the Public on the concepts behind Synthetic Biology.
- The exhibit attracts a diverse audience from school kids to science enthusiasts.
- I have started to design a hands-on activity, and describe its early design below.
Intentions behind the activity
- To set the background in terms of Molecular and Celluar Biology:
- DNA, Central Dogma, Cells and Bacteria
- Genetic Engineering techniques
- To touch on Synthetic Biology concepts:
- BioBricks
- Modularity
- Re-usability
- To give an opportunity to build simple genetic circuits based on opto-electronic components:
- Protein Generator
- Inverter, Toggle switch & Repressilator
- Link circuits with inputs such as: temperature sensor, Infra-red sensor, Touch sensor, Humidity sensor.
- Link circuits with outputs such as: flagella motor, secretion fan.
Overview
Components and Schematics
Basic Gene Expression Circuits
Protein Generator | Fusion Protein Generator | Monocystronic Protein Generator |
Elementary Genetic Circuits
Inverter | Repressillator |