User:Vincent Rouilly/Hands On BioBrick Activity: Difference between revisions
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| [[Image:HandsOnBioBrick_FusionProteinGenerator.png|300px|Activated Promoter Component]] | | [[Image:HandsOnBioBrick_FusionProteinGenerator.png|300px|Activated Promoter Component]] | ||
| [[Image:HandsOnBioBrick_MonocystronicProteinGenerator.png|300px|Repressed Promoter Component]] | | [[Image:HandsOnBioBrick_MonocystronicProteinGenerator.png|300px|Repressed Promoter Component]] | ||
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* the protein expression level is tuned by the potentiometers of the promoter and the RBS | |||
* the protein expression level can be picked up with a fiber optic to drive another promoter or an output modules (flagella motor or secretion fan). | |||
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* the expression level of both proteins is tuned by the potentiometers of the promoter and the RBS. It is the same for both proteins. | |||
* the protein expression levels can be picked up with a fiber optic to drive another promoter or an output modules (flagella motor or secretion fan). | |||
| | |||
* control over the protein expression levels is tuned by the potentiometer of the promoter, but also, independently controlled by the potentiometers of the RBSs. | |||
* the protein expression levels can be picked up with a fiber optic to drive another promoter or an output modules (flagella motor or secretion fan). | |||
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Revision as of 02:47, 7 June 2007
Vincent 13:42, 6 June 2007 (EDT): feel free to comment on the content of the activity described and its design.
Context
- Following last year | Imperial College participation to iGEM, my group is putting together an exhibit for the Royal Society Summer Science Exhibition 2007.
- We feel it is a great opportunity to engage with the Public on the concepts behind Synthetic Biology.
- The exhibit attracts a diverse audience from school kids to science enthusiasts.
- I have started to design a hands-on activity, and describe its early design below.
Intentions behind the activity
- To set the background in terms of Molecular and Celluar Biology:
- DNA, Central Dogma, Cells and Bacteria
- Genetic Engineering techniques
- To touch on Synthetic Biology concepts:
- BioBricks
- Modularity
- Re-usability
- To give an opportunity to build simple genetic circuits based on opto-electronic components:
- Protein Generator
- Inverter, Toggle switch & Repressilator
- Link circuits with inputs such as: temperature sensor, Infra-red sensor, Touch sensor, Humidity sensor.
- Link circuits with outputs such as: flagella motor, secretion fan.
Overview
Components and Schematics
Basic Gene Expression Circuits
Elementary Genetic Circuits
Inverter | Repressillator |
Material
Biobricks
- constitutive promoter:
- Duplo brick 2x2
- potentiometer
- connector
- battery
- electronic circuit
- activated/repressed promoter:
- ribosome binding site
- Duplo brick 2x2
- potentiometer to tune strength of RBS
- battery
- connectors x2
- Duplo brick 2x2
- Protein coding region
- Duplo brick 4x2
- LED (with different colors): LEDs
- multi level bar graph LED + electronic circuit: bar graph display, bar graph driver
- connectors x2
- Stop codon
- Duplo brick 2x2
- Connector x1
Inputs Sensors
Motors
Electronic Circuits
- Light/Dark detector
- temperature AOP
- light sensor based on transistors
- optic fibre transmitter
- good stuff
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