User:Vincent Rouilly/Hands On BioBrick Activity

Vincent 13:42, 6 June 2007 (EDT): feel free to comment on the content of the activity described and its design.

Context

• Following last year Imperial College participation to iGEM, my group is putting together an exhibit for the Royal Society Summer Science Exhibition 2007.
• We feel it is a great opportunity to engage with the Public on the concepts behind Synthetic Biology.
• The exhibit attracts a diverse audience from school kids to science enthusiasts.
• I have started to design a hands-on activity, and describe its early design below.

Intentions behind the activity

• To set the background in terms of Molecular and Celluar Biology:
  • DNA, Central Dogma, Cells and Bacteria
  • Genetic Engineering techniques
• To touch on Synthetic Biology concepts:
  • BioBricks
  • Modularity
  • Re-usability
• To give an opportunity to build simple genetic circuits based on opto-electronic components:
  • Protein Generator
  • Inverter, Toggle switch & Repressilator
  • Link circuits with inputs such as: temperature sensor, Infra-red sensor, Touch sensor, Humidity sensor.
  • Link circuits with outputs such as: flagella motor, secretion fan.

Overview

Overview	General setting
the activity stands on an oval table, decorated as an E.Coli bacteria. on the right side of the table, DNA, gene expression machinery are illustrated. the hands on activity is placed on the left side: a set of available biobricks implemented as Lego bricks filled with some opto-electronic components. a conductive surface a set of fiber optics to link the different genetic circuits.	the activity offers a set of possible inputs for the genetic circuits to be built: temperature sensor (to illustrate heat shock response). infra-red sensor - with remote - (to illustrate UV response) touch sensor humidity sensor 2 outputs are also available: motor driving some flagella fan to illustrate secretion

Components and Schematics

Constitutive Promoter	Activated Promoter	Repressed Promoter
Strength of the promoter is set through the potentiometer A battery is embedded into the lego brick The Lego brick is sealed with a conductive plate to make contact with the table.	Activity of the promoter is driven by the amount of light received on the photo-sensor. A battery is embedded into the lego brick The Lego brick is sealed with a conductive plate to make contact with the table.	Activity of the promoter is driven by the amount of light received on the photo-sensor. A battery is embedded into the lego brick The Lego brick is sealed with a conductive plate to make contact with the table.
RBS	Protein Coding Region	Stop Codon
Strength of the RBS is set through the potentiometer	a 10-LEDs graph bar is used to indicate the level of expression. a LED is used separately to potentially drive another promoter through a fiber optic. the Resistor-Capacitor is used to add a delay.	The stop codon enables the circuit to connect to earth through the table

Basic Gene Expression Circuits

Protein Generator	Fusion Protein Generator	Monocystronic Protein Generator
the protein expression level is tuned by the potentiometers of the promoter and the RBS the protein expression level can be picked up with a fiber optic to drive another promoter or an output modules (flagella motor or secretion fan).	the expression level of both proteins is tuned by the potentiometers of the promoter and the RBS. It is the same for both proteins. the protein expression levels can be picked up with a fiber optic to drive another promoter or an output modules (flagella motor or secretion fan).	control over the protein expression levels is tuned by the potentiometer of the promoter, but also, independently controlled by the potentiometers of the RBSs. the protein expression levels can be picked up with a fiber optic to drive another promoter or an output modules (flagella motor or secretion fan).

Elementary Genetic Circuits

Inverter	Repressillator

Material

Biobricks

• constitutive promoter:
  • Duplo brick 2x2
  • potentiometer
  • connector
  • battery
  • electronic circuit

• activated/repressed promoter:
  • Duplo brick 2x2
  • LED sensor: sensor1
  • connector connector others others
  • battery
  • electronic circuit

• ribosome binding site
  • Duplo brick 2x2
    • potentiometer to tune strength of RBS
    • battery
  • connectors x2

• Protein coding region
  • Duplo brick 4x2
  • LED (with different colors): LEDs
  • multi level bar graph LED + electronic circuit: bar graph display, bar graph driver
  • connectors x2

• Stop codon
  • Duplo brick 2x2
  • Connector x1

Inputs Sensors

• Temperature:kit
• Touch: kit
• Infra-red: kit
• Humidity: kit

Motors

• motor: motors
• fans: fans

Electronic Circuits

• Light/Dark detector
• temperature AOP
• light sensor based on transistors
• optic fibre transmitter
• good stuff

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