User:Vincent Rouilly/Hands On BioBrick Activity
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Vincent 13:42, 6 June 2007 (EDT): feel free to comment on the content of the activity described and its design.
Context
- Following last year Imperial College participation to iGEM, my group is putting together an exhibit for the Royal Society Summer Science Exhibition 2007.
- We feel it is a great opportunity to engage with the Public on the concepts behind Synthetic Biology.
- The exhibit attracts a diverse audience from school kids to science enthusiasts.
- I have started to design a hands-on activity, and describe its early design below.
Intentions behind the activity
- To set the background in terms of Molecular and Celluar Biology:
- DNA, Central Dogma, Cells and Bacteria
- Genetic Engineering techniques
- To touch on Synthetic Biology concepts:
- BioBricks
- Modularity
- Re-usability
- To give an opportunity to build simple genetic circuits based on opto-electronic components:
- Protein Generator
- Inverter, Toggle switch & Repressilator
- Link circuits with inputs such as: temperature sensor, Infra-red sensor, Touch sensor, Humidity sensor.
- Link circuits with outputs such as: flagella motor, secretion fan.
Overview
Components and Schematics
Basic Gene Expression Circuits
Elementary Genetic Circuits
Inverter | Repressillator |
Material
Biobricks
- constitutive promoter:
- Duplo brick 2x2
- potentiometer
- connector
- battery
- electronic circuit
- activated/repressed promoter:
- ribosome binding site
- Duplo brick 2x2
- potentiometer to tune strength of RBS
- battery
- connectors x2
- Duplo brick 2x2
- Protein coding region
- Duplo brick 4x2
- LED (with different colors): LEDs
- multi level bar graph LED + electronic circuit: bar graph display, bar graph driver
- connectors x2
- Stop codon
- Duplo brick 2x2
- Connector x1
Inputs Sensors
Motors
Electronic Circuits
- Light/Dark detector
- temperature AOP
- light sensor based on transistors
- optic fibre transmitter
- good stuff
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