User:Wesley J. Houston/Notebook/MAP/2013/02/08

(Difference between revisions)

Revision as of 02:11, 7 February 2013

Building a Reporter Gene

Assembly: 5xGal4 : [Spacer : HSVtk TATA] : K : AmCyan : AmCyan : NLS : STOP : [polyA2] KAH201/KAH182; AmCyan reporter gene for chromatin proteins.

Assemblies

> Digests (Fermentas FD)

> Measure conc.'s of gel purified fragments

> Ligations (edit after ligation is done)

Ligation	Plate results (lig : neg crtl) mm/dd/yy
1. KAH201 (E/S)/203, x ng + KAH182 (E/X)/1675, x ng	---
2. KAH182 (E/X)/ x ng	---

@@ Line 12: / Line 12: @@
 ----
 '''Assemblies'''
-# KAH201/KAH182: (1) KAH201/(X/P)/203 + (2) KAH182/(S/P)/1675
+# KAH201/KAH182: (1) KAH201/(E/S)/203 + (2) KAH182/(E/X)/1675
 > Digests (Fermentas FD)<br>
@@ Line 41: / Line 41: @@
 | <u>Sample</u> || <u>OD260</u> || <u>260/280</u> || <u>ng/μL</u>
 |-
-| 1. KAH201 (X/P) || TBD || TBD || TBD
+| 1. KAH201 (E/S) || TBD || TBD || TBD
 |-
-| 2. KAH182 (S/P) || TBD || TBD || TBD
+| 2. KAH182 (E/X) || TBD || TBD || TBD
 |}
@@ Line 51: / Line 51: @@
 | <u>Ligation</u> || <font color="blue"><u>Plate results (lig : neg crtl)</u> mm/dd/yy</font>
 |-
-| 1. KAH201 (X/P)/203, x ng + KAH182 (S/P)/1675, x ng || <font color="blue">---</font>
+| 1. KAH201 (E/S)/203, x ng + KAH182 (E/X)/1675, x ng || <font color="blue">---</font>
 |-
-| 2. KAH182 (S/P)/ x ng || ---
+| 2. KAH182 (E/X)/ x ng || ---
 |}