User:Wesley J. Houston/Notebook/MAP/2013/02/08: Difference between revisions
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| <u>Ligation</u> || <font color="blue"><u>Plate results (lig : neg crtl)</u> mm/dd/yy</font> | | <u>Ligation</u> || <font color="blue"><u>Plate results (lig : neg crtl)</u> mm/dd/yy</font> | ||
|- | |- | ||
| 1. KAH201 (S/P)/203, | | 1. KAH201 (S/P)/203, 25 ng + KAH182 (X/P)/1675, 6 ng || <font color="blue">TBD</font> | ||
|- | |- | ||
| 2. KAH182 (X/P)/1675 | | 2. KAH182 (X/P)/1675 6 ng || TBD | ||
|} | |} | ||
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| || 1 || 2 || | | || 1 || 2 || | ||
|- | |- | ||
| Insert DNA || | | Insert DNA || 0.74 || --- || | ||
|- | |- | ||
| Vector DNA || 0. | | Vector DNA || 0.52 || 0.52 || | ||
|- | |- | ||
| 2x lgn buf (Roche) || 5.0 || 5.0 || | | 2x lgn buf (Roche) || 5.0 || 5.0 || | ||
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| T4 ligase (NEB) || 1.0 || 1.0 || | | T4 ligase (NEB) || 1.0 || 1.0 || | ||
|- | |- | ||
| dH<sub>2</sub>O || | | dH<sub>2</sub>O || 2.74 || 3.48 || | ||
|- | |- | ||
| || 10 μL || 10 μL || | | || 10 μL || 10 μL || |
Revision as of 13:59, 21 February 2013
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February 8, 2013
Assemblies
> Digests (Fermentas FD)
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