User:Wesley J. Houston/Notebook/MAP/2013/02/08: Difference between revisions
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---- | ---- | ||
'''Assemblies''' | '''Assemblies''' | ||
# KAH201/KAH182: (1) KAH201/( | # KAH201/KAH182: (1) KAH201/(X/P)/203 + (2) KAH182/(S/P)/1675 | ||
> Digests (Fermentas FD)<br> | > Digests (Fermentas FD)<br> | ||
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| rowspan="9" | [[Image:KAH113012_gel1.jpg |150px|Hover name]]<br>30 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | | rowspan="9" | [[Image:KAH113012_gel1.jpg |150px|Hover name]]<br>30 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
|- | |- | ||
| DNA (plasmid) || | | DNA (plasmid) || 15 to 25 μL | ||
|- | |- | ||
| 10x buffer || 3.0 | | 10x buffer || 3.0 | ||
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| enzyme 2 || 1.0 | | enzyme 2 || 1.0 | ||
|- | |- | ||
| dH<sub>2</sub>O || | | dH<sub>2</sub>O || 0 to 10 μL | ||
|- | |- | ||
| || 30 μL --> 37°C/ ~15 min. | | || 30 μL --> 37°C/ ~15 min. | ||
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| <u>Sample</u> || <u>OD260</u> || <u>260/280</u> || <u>ng/μL</u> | | <u>Sample</u> || <u>OD260</u> || <u>260/280</u> || <u>ng/μL</u> | ||
|- | |- | ||
| 1. KAH201 ( | | 1. KAH201 (X/P) || TBD || TBD || TBD | ||
|- | |- | ||
| 2. KAH182 ( | | 2. KAH182 (S/P) || TBD || TBD || TBD | ||
|} | |} | ||
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| <u>Ligation</u> || <font color="blue"><u>Plate results (lig : neg crtl)</u> 11/31/12</font> | | <u>Ligation</u> || <font color="blue"><u>Plate results (lig : neg crtl)</u> 11/31/12</font> | ||
|- | |- | ||
| 1. KAH201 ( | | 1. KAH201 (X/P)/203, x ng + KAH182 (S/P)/1675, x ng || <font color="blue">---</font> | ||
|- | |- | ||
| 2. KAH182 ( | | 2. KAH182 (S/P)/ 25 ng || --- | ||
|} | |} | ||
Revision as of 22:47, 6 February 2013
Building a Reporter Gene | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
February 8, 2013
Assemblies
> Digests (Fermentas FD)
|