User:Wesley J. Houston/Notebook/MAP/2013/02/28

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(2/28/13)
 
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==2/28/13==
==2/28/13==
* Prep plasmid DNA again, using Zyppy plasmid kit.
* Prep plasmid DNA again, using Zyppy plasmid kit.
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* Digest using restriction enzymes.
+
* Determined concentrations of KAH182 and KAH201.
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** KAH201/(S/P)/203
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** KAH182/(X/P)/1675
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{| class="wikitable" border="0" cellspacing="3" <!-- [DNA] table -->
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|-
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| <u>Sample</u> || <u>260/280</u> || <u>ng/μL</u>
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|-
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| 1. KAH201 (Plasmid) || 1.751 || 198.766
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|-
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| 2. KAH182 (Plasmid) || 1.285 || 19.685
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|}
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* Very low concentration and purity of KAH182
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2/28/13

  • Prep plasmid DNA again, using Zyppy plasmid kit.
  • Determined concentrations of KAH182 and KAH201.
Sample 260/280 ng/μL
1. KAH201 (Plasmid) 1.751 198.766
2. KAH182 (Plasmid) 1.285 19.685
  • Very low concentration and purity of KAH182


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