User:Wesley J. Houston/Notebook/MAP/2013/03/07: Difference between revisions
From OpenWetWare
(→3/7/13) |
(→3/7/13) |
||
Line 8: | Line 8: | ||
==3/7/13== | ==3/7/13== | ||
* Prepped plasmids using Zyppy miniprep kit. | * Prepped plasmids using Zyppy miniprep kit. | ||
* Digested plasmids with EcoR1 and Pst1 | * Digested plasmids with EcoR1 and Pst1. | ||
* Ran gel electrophoresis to confirm assembly ( | * Ran gel electrophoresis to confirm assembly. | ||
* No DNA present, repeat ligation. | |||
{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table --> | |||
|-valign="top" | |||
| <u>Reagent</u> || <u>Volume</u> || | |||
| rowspan="9" | [[Image:Electrophoresis3.jpg |150px|Results of the ligation electrophoresis]]<br>15 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | |||
|- | |||
| DNA (plasmid) || 20 μL | |||
|- | |||
| 10x buffer || 3.0 | |||
|- | |||
| enzyme 1 || 1.0 | |||
|- | |||
| enzyme 2 || 1.0 | |||
|- | |||
| dH<sub>2</sub>O || 5.0 μL | |||
|- | |||
| || 30 μL --> 37°C/ ~15 min. | |||
|} | |||
* No DNA present, will repeat ligation. | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 13:17, 18 March 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||
3/7/13
|