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3/7/13
- Prepped plasmids using Zyppy miniprep kit.
- Digested plasmids with EcoR1 and Pst1.
- Ran gel electrophoresis to confirm assembly.
| Reagent |
Volume |
|
15 μL/lane, 1% agarose; Ladder
|
| DNA (plasmid) |
20 μL
|
| 10x buffer |
3.0
|
| enzyme 1 |
1.0
|
| enzyme 2 |
1.0
|
| dH2O |
5.0 μL
|
| |
30 μL --> 37°C/ ~15 min.
|
- No DNA present, will repeat ligation.
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