User:Wesley J. Houston/Notebook/MAP/2013/03/18
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==3/18/2013== | ==3/18/2013== | ||
* Retrying ligation of KAH201(S/P)/3403 + KAH182(X/P)/1675 | * Retrying ligation of KAH201(S/P)/3403 + KAH182(X/P)/1675 | ||
| + | * Concentrations: KAH201 = 48.1 ng/uL, KAH1182 = 6.3 ng/uL | ||
| + | ** Concentrations were determined on 2/18/13 | ||
| - | > Ligations, following corrected version from | + | > Ligations, following corrected version from 3/4/13, with 50 ng of vector instead of 25 ng. |
{| class="wikitable" border="0" cellspacing="3" <!-- Ligations table --> | {| class="wikitable" border="0" cellspacing="3" <!-- Ligations table --> | ||
| <u>Ligation</u> || <font color="blue"><u>Plate results (lig : neg crtl)</u> 03/18/13</font> | | <u>Ligation</u> || <font color="blue"><u>Plate results (lig : neg crtl)</u> 03/18/13</font> | ||
| Line 21: | Line 23: | ||
| || 1 || 2 || | | || 1 || 2 || | ||
|- | |- | ||
| - | | Insert DNA (KAH182)|| | + | | Insert DNA (KAH182)|| 8.0 || --- || |
|- | |- | ||
| Vector DNA (KAH201)|| 1.0 || 1.0 || | | Vector DNA (KAH201)|| 1.0 || 1.0 || | ||
|- | |- | ||
| - | | 2x lgn buf (Roche) || | + | | 2x lgn buf (Roche) || 10.0 || 5.0 || |
|- | |- | ||
| T4 ligase (NEB) || 1.0 || 1.0 || | | T4 ligase (NEB) || 1.0 || 1.0 || | ||
| Line 31: | Line 33: | ||
| dH<sub>2</sub>O || 0 || 3.0 || | | dH<sub>2</sub>O || 0 || 3.0 || | ||
|- | |- | ||
| - | | || | + | | || 20 μL || 10 μL || |
|} | |} | ||
Revision as of 15:38, 18 March 2013
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3/18/2013
> Ligations, following corrected version from 3/4/13, with 50 ng of vector instead of 25 ng.
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