User:Wesley J. Houston/Notebook/MAP/2013/03/21

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(Autocreate 2013/03/21 Entry for User:Wesley_J._Houston/Notebook/MAP)
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==Entry title==
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==3/21/13==
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* Retrieved liquid cultures from the incubator.
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* Pelleted the samples (3 mins @ 16.3k RPM).
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** No pellets of KAH201 were produced.
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** KAH182 was successfully pelleted.
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* Next, I followed the established protocol for the Zyppy plasmid prep kit and made plasmids of KAH182.
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** Concentration and purity was also determined (43.201, 1.758 (1); 40.434, 1.751(2)).
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*** Note there were two samples of KAH182.
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* Since the previously made KAH201 colonies (made on Jan 24, 2013) appear to not be viable, I have also made more KAH201 colonies.
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** Following the established protocol for bacterial transformation.

Revision as of 17:46, 21 March 2013

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3/21/13

  • Retrieved liquid cultures from the incubator.
  • Pelleted the samples (3 mins @ 16.3k RPM).
    • No pellets of KAH201 were produced.
    • KAH182 was successfully pelleted.
  • Next, I followed the established protocol for the Zyppy plasmid prep kit and made plasmids of KAH182.
    • Concentration and purity was also determined (43.201, 1.758 (1); 40.434, 1.751(2)).
      • Note there were two samples of KAH182.
  • Since the previously made KAH201 colonies (made on Jan 24, 2013) appear to not be viable, I have also made more KAH201 colonies.
    • Following the established protocol for bacterial transformation.



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