User:Wesley J. Houston/Notebook/MAP/2013/03/21: Difference between revisions

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==3/21/13==
==3/21/13==
* Retrieved liquid cultures from the incubator.
* Retrieved liquid cultures from the incubator.
* Pelleted the samples (3 mins @ 16.3k RPM).
* Pelleted the samples (3 mins @ 16.3k x g).
** No pellets of KAH201 were produced.
** No pellets of KAH201 were produced.
** KAH182 was successfully pelleted.
** KAH182 was successfully pelleted.

Revision as of 15:24, 25 March 2013

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3/21/13

  • Retrieved liquid cultures from the incubator.
  • Pelleted the samples (3 mins @ 16.3k x g).
    • No pellets of KAH201 were produced.
    • KAH182 was successfully pelleted.
  • Next, I followed the established protocol for the Zyppy plasmid prep kit and made plasmids of KAH182.
    • Concentration and purity was also determined (43.201, 1.758 (1); 40.434, 1.751(2)).
      • Note there were two samples of KAH182.
  • Since the previously made KAH201 colonies (made on Jan 24, 2013) appear to not be viable, I have also made more KAH201 colonies.
    • Following the established protocol for bacterial transformation.



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