User:Wesley J. Houston/Notebook/MAP/2013/03/22

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3/22/13

Reagent Volume   Results of third electrophoresis
15 μL/lane, 1% agarose; Ladder
DNA (plasmid) 20 μL
10x buffer 3.0
enzyme 1 (Pst1) 1.0
enzyme 2 (Xba1 or Spe1) 1.0
dH2O 5.0 μL
  30 μL --> 37°C/ ~15 min.


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