User:Wesley J. Houston/Notebook/MAP/2013/03/25: Difference between revisions

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| &nbsp; || 30 μL --> 37°C/ ~15 min.
| &nbsp; || 30 μL --> 37°C/ ~15 min.
|}
|}
> Measure conc.'s of gel purified fragments
{| class="wikitable" border="0" cellspacing="3" <!-- [DNA] table -->
|-
| <u>Sample</u> || <u>260/280</u> || <u>ng/μL</u>
|-
| 1. KAH201 (S/P) || 0.727 || 2.54
|-
| 2. KAH182 (X/P) || 0.789 || 13596
|}


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Revision as of 21:55, 25 March 2013

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3/25/13

Reagent Volume   Results of the fourth electrophoresis attempt
15 μL/lane, 1% agarose; Ladder
DNA (plasmid) 25 μL
10x buffer 3.0
enzyme 1 (Pst1) 1.0
enzyme 2 (Xba1 or Spe1) 1.0
dH2O 0.0 μL
  30 μL --> 37°C/ ~15 min.

> Measure conc.'s of gel purified fragments

Sample 260/280 ng/μL
1. KAH201 (S/P) 0.727 2.54
2. KAH182 (X/P) 0.789 13596



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