User:Wesley J. Houston/Notebook/MAP/2013/03/26: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==3/26==
==3/26/13==
* Prepped plasmids of KAH182 and KAH201 liquid cultures, following the Zyppy plasmid miniprep kit.
* Prepped plasmids of KAH182 and KAH201 liquid cultures, following the Zyppy plasmid miniprep kit.
* Determined yields of plasmids.
* Determined yields of plasmids.


{| class="wikitable" border="0" cellspacing="3" <!-- [DNA] table -->
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| 2. KAH201 (Plasmid) || 0.069 || 1.857 || 69.164
| 2. KAH201 (Plasmid) || 0.069 || 1.857 || 69.164
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> Measure conc.'s of gel purified fragments
 
> Measure conc.'s of gel purified fragments (KAH182 = lanes 2 and 3; KAH201 = lanes 4 and 5)
{| class="wikitable" border="0" cellspacing="3" <!-- [DNA] table -->
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Latest revision as of 22:35, 26 September 2017

Project name Main project page
Previous entry      Next entry

3/26/13

  • Prepped plasmids of KAH182 and KAH201 liquid cultures, following the Zyppy plasmid miniprep kit.
  • Determined yields of plasmids.


Sample OD260 260/280 ng/μL
1. KAH201 (Plasmid) 0.056 1.732 56.395
2. KAH201 (Plasmid) 0.069 1.857 69.164


Reagent Volume   Results of the fifth electrophoresis attempt
30 μL/lane, 1% agarose; Ladder
DNA (plasmid) 25 μL
10x buffer 3.0
enzyme 1 (Pst1) 1.0
enzyme 2 (Xba1 or Spe1) 1.0
dH2O 0.0 μL
  30 μL --> 37°C/ ~15 min.


> Measure conc.'s of gel purified fragments (KAH182 = lanes 2 and 3; KAH201 = lanes 4 and 5)

Sample OD260 260/280 ng/μL
1. KAH201 (S/P) 0.029 1.054 29.262
2. KAH201 (S/P) 0.006 1.381 6.172
3. KAH182 (X/P) -0.002 2 -1.702
4. KAH182 (X/P) 0.007 5.583 7.129



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