User:Wesley J. Houston/Notebook/MAP/2013/03/26

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3/26/13

  • Prepped plasmids of KAH182 and KAH201 liquid cultures, following the Zyppy plasmid miniprep kit.
  • Determined yields of plasmids.


Sample OD260 260/280 ng/μL
1. KAH201 (Plasmid) 0.056 1.732 56.395
2. KAH201 (Plasmid) 0.069 1.857 69.164


Reagent Volume   Results of the fifth electrophoresis attempt
30 μL/lane, 1% agarose; Ladder
DNA (plasmid) 25 μL
10x buffer 3.0
enzyme 1 (Pst1) 1.0
enzyme 2 (Xba1 or Spe1) 1.0
dH2O 0.0 μL
  30 μL --> 37°C/ ~15 min.


> Measure conc.'s of gel purified fragments (KAH182 = lanes 2 and 3; KAH201 = lanes 4 and 5)

Sample OD260 260/280 ng/μL
1. KAH201 (S/P) 0.029 1.054 29.262
2. KAH201 (S/P) 0.006 1.381 6.172
3. KAH182 (X/P) -0.002 2 -1.702
4. KAH182 (X/P) 0.007 5.583 7.129



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