User:Wesley J. Houston/Notebook/MAP/2013/03/26

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Autocreate 2013/03/26 Entry for User:Wesley_J._Houston/Notebook/MAP)
(Entry title)
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
==Entry title==
+
==3/26==
-
* Insert content here...
+
* Prepped plasmids of KAH182 and KAH201 liquid cultures, following the Zyppy plasmid miniprep kit.
 +
* Determined yields of plasmids.
 +
 
 +
{| class="wikitable" border="0" cellspacing="3" <!-- [DNA] table -->
 +
|-
 +
| <u>Sample</u> || <u>OD260</u> || <u>260/280</u> || <u>ng/μL</u>
 +
|-
 +
| 1. KAH201 (Plasmid) || 0.056 || 1.732 || 56.395
 +
|-
 +
| 2. KAH201 (Plasmid) || 0.069 || 1.857 || 69.164
 +
|}
 +
 
 +
{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table -->
 +
|-valign="top"
 +
| <u>Reagent</u> || <u>Volume</u> || &nbsp;
 +
| rowspan="9" | [[Image:Electrophoresis6.jpg‎ |150px|Results of the fifth electrophoresis attempt]]<br>30 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
 +
|-
 +
| DNA (plasmid) || 25 μL
 +
|-
 +
| 10x buffer || 3.0
 +
|-
 +
| enzyme 1 (Pst1) || 1.0
 +
|-
 +
| enzyme 2 (Xba1 or Spe1)|| 1.0
 +
|-
 +
| dH<sub>2</sub>O || 0.0 μL
 +
|-
 +
| &nbsp; || 30 μL --> 37°C/ ~15 min.
 +
|}
 +
 
 +
> Measure conc.'s of gel purified fragments
 +
{| class="wikitable" border="0" cellspacing="3" <!-- [DNA] table -->
 +
|-
 +
| <u>Sample</u> || <u>OD260</u> || <u>260/280</u> || <u>ng/μL</u>
 +
|-
 +
| 1. KAH201 (S/P) || 0.029 || 1.054 || 29.262
 +
|-
 +
| 2. KAH201 (S/P) || 0.006 || 1.381 || 6.172
 +
|-
 +
| 3. KAH182 (X/P) || -0.002 || 2 || -1.702
 +
|-
 +
| 4. KAH182 (X/P) || 0.007 || 5.583 || 7.129
 +
|}

Revision as of 14:20, 31 March 2013

Project name Main project page
Previous entry      Next entry

3/26

  • Prepped plasmids of KAH182 and KAH201 liquid cultures, following the Zyppy plasmid miniprep kit.
  • Determined yields of plasmids.
Sample OD260 260/280 ng/μL
1. KAH201 (Plasmid) 0.056 1.732 56.395
2. KAH201 (Plasmid) 0.069 1.857 69.164
Reagent Volume   Results of the fifth electrophoresis attempt
30 μL/lane, 1% agarose; Ladder
DNA (plasmid) 25 μL
10x buffer 3.0
enzyme 1 (Pst1) 1.0
enzyme 2 (Xba1 or Spe1) 1.0
dH2O 0.0 μL
  30 μL --> 37°C/ ~15 min.

> Measure conc.'s of gel purified fragments

Sample OD260 260/280 ng/μL
1. KAH201 (S/P) 0.029 1.054 29.262
2. KAH201 (S/P) 0.006 1.381 6.172
3. KAH182 (X/P) -0.002 2 -1.702
4. KAH182 (X/P) 0.007 5.583 7.129



Personal tools