User:Wesley J. Houston/Notebook/MAP/2013/04/04

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4/4/13

  • The liquid cultures did not grow.
  • Prepped two more liquid cultures from the ligation plate, as well as performed another ligation.

> Ligation

  1 2
Insert DNA (KAH182) 7.0 ---
Vector DNA (KAH201) 1.7 1.7
2x lgn buf (Roche) 9.7 9.7
T4 ligase (NEB) 1.0 1.0
dH2O 0.6 7.6
  20 μL 20 μL
  • Follow quick transformation protocol
  • Add 30 uL DH5α-Turbo to each ligation; plate on 100 μg/mL Amp agar
Ligation Plate results (lig : neg crtl)04/01/13: Ligation
1. KAH201 (S/P)/3403, 50 ng + KAH182 (X/P)/1675, 50 ng 0
2. KAH201 (S/P)/3403, 50 ng 0
  • The ligation was unsuccessful.


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