User:Whitney V. Kyle/Notebook/Biology 210 at AU

2/3/16

OWW entry #1

Observation of Hay Infusion While looking at our hay infusion, I observed that the smell was not as bad as last week. The infusion also looked significantly murkier, and there was more sediment buildup on the bottom of the jar. I think this is because the things like leaves have had more time to breakdown, which account for the buildup at the bottom and the murkiness. I also think since the jar was open, a lot of the water evaporated, which makes the infusion more concentrated with bacteria, leaves, sticks, etc. This may be why the smell is better because it has had time to air out. There are no Archaea because the infusion's environment is not extreme.

Dilution, agar, #Colonies on Plate, Colonies/ml

10-3, nutrient, 300, 300,000 10-5, nutrient, 150, 15000000 10-7,nutrient,940000000 10-9,nutreint,78,7.8e10

10-3,nutrient+tet,28,28000 10-5,nutrient+tet,0,0 10-7,nutrient+tet,0,0 10-9,nutrient+tet,0,0

OWW entry #2 There is an observable difference between the plates with versus without the antibiotic. All of the Tet plus plates except for 10-3 were not resistant against tetracycline.The effect of tetracycline is reflected in a decrease in the total number of bacteria and fungi.

OWW entry #3 Bacteria that are sensitive to tetracycline are chlamidya, rickettsia, and spirochete. Mechanism of Action: Antibiotics from tetracycline are protein synthesis inhibitors, which inhibits the binding of ammoniacal-trna to the mana-ribosome complex. This happens by binding the to the 30S ribosomal subunit in the MRNA translation complex.

Chopra, I., & Roberts, M. (2001). Tetracycline Antibiotics: Mode of Action, Applications, Molecular Biology, and Epidemiology of Bacterial Resistance. Microbiology and Molecular Biology Reviews, 65(2), 232–260. http://doi.org/10.1128/MMBR.65.2.232-260.2001

OWW entry #4

Colony Label, Plate Type, Colony Description, Cell Description

Coccus, 10-3 tet+, 2.5um purple circular, nonmotile and uniform Bacillus, 10-9, purple 2.5 um line, moving quickly and uniform Coccus, 10-9, purple/blue >1um circular, nonmotile and uniform Coccus, 10-3 tet+, >1um purple circular, nonmotile and uniform

Transect #4

Abiotic Factors: - benches - rocks - bird feeder - wildlife habitat sign - pond - animal tracks - drain - statues - stone path -trash cans -snow -artificial light -roper building

Biotic Factors -tree -squirrels -birds -bushes -plant life -human interaction

Transect #4 is a Wildlife Habitat, certified by the National Wildlife Federation. This means that our transect provides the four basic habitat elements needed for wildlife to thrive: food, water, cover, and places to raise young. From our observations of the transect, food has been provided (bird feeder, acorns, plants, etc), water (the pond even though it is covered and frozen over), cover (the trees, small spaces,) and places to raise their young (the transect itself). There is an abundance of unidentified plant life in our transect including a few types of trees, grass, thorn bushes, and flowers. Our transect is somewhat shady because of the trees, but in full sun even the small plants get some degree of sunlight. Another interesting thing we found are animal tracks which we suspect were made from deer which we have seen roaming around campus.