User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization

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====Protocols====
====Protocols====
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** [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/15#Method| Preparation of Medium]]
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* [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/03/26#Plating_cells_from_-80_.C2.B0C| Taking cells from -80 °C stock]]
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** [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/10| Splitting cells]]
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* [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/15#Method| Preparation of Medium]]
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** [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/12| Counting cells]]
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** [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/03/26#Preparation_of_Heat_inactivated_FBS.2FFCS|Preparation Heat inactivated FBS]]
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** [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/16| Cell stimulation]]
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* [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/10| Splitting cells]]
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** [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/17| Viability staining]]
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* [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/12| Counting cells]]
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** [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/18| ELISA]] ([[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/25|Coating buffer]])
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* [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/16| Cell stimulation]]
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** Co-immunoprecipitation [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/23| Part I]],  [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/24| Part II]] & [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/25| Part III]]
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* [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/17| Viability staining]]
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** [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/24|Pierce determination]] (protein conc.)
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* [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/18| ELISA]] ([[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/25|Coating buffer]])
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** [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/03/01| Western blot]]
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* Co-immunoprecipitation [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/23| Part I]],  [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/24| Part II]] & [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/25| Part III]]
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* [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/24|Pierce determination]] (protein conc.)
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* [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/03/01| Western blot]]
====Results====
====Results====

Revision as of 08:37, 26 March 2010

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Project Description/Abstract

“Towards insights into cyclic AMP compartmentalization in cell of lung origin” a cooperation between the department of Molecular Pharmacology (University Groningen, the Netherlands) and the Leibniz-Institut für Molekulare Pharmakologie (Berlin, Germany). These are the entries of a Master internship from February 'till September 2010.

Notes

Interesting entries

Protocols

Results

ELISA
  1. Viability & ELISA
  2. Dose effect curve ELISA
  3. ELISA
  4. ELISA
Co-IP
  1. Co-IP 1 Anouk
  2. Co-IP 2 Anouk
  3. Co-IP 3 Anouk
  4. Co-IP 4TODO
Misc.

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Table of contents

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Groningen Berlin

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