User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization

ELISA

1. Viability & ELISA
2. Dose effect curve ELISA
3. ELISA
4. ELISA

Co-IP

1. Co-IP 1 Anouk
2. Co-IP 2 Anouk
3. Co-IP 3 Anouk
4. Co-IP 4 TODO

Misc.

• Ht31P toxicity
• Statistical analysis

• 10/02/2010: Splitting ASM cells
• 12/02/2010: Counting cells
• 15/02/2010: Putting cells to S⁰
• 16/02/2010: Stimulation hTERT cells
• 17/02/2010: Viability staining
• 18/02/2010: ELISA
• 19/02/2010: Counting and splitting cells
• 22/02/2010: Preparing new DMEM S⁺
• 23/02/2010: Putting cells to S⁰, Co-immunoprecipitation
• 24/02/2010: Co-immunoprecipitation part II, (Stimulation hTERT cells v2)
• 25/02/2010: Co-immunoprecipitation part III & Viability staining RESULTS
• 26/02/2010: ELISA, counting/splitting cells RESULTS
• 01/03/2010: Western blot RESULTS
• 03/03/2010: Co-IP day I, GAPDH, VASP, Stop Stimulation HT4 TODO
• 04/03/2010: Co-IP day II, ELISA HT4, Cell Stimulation TODO
• 05/03/2010: Co-IP day III, Stop stimulation hTERT, Split hTERT TODO
• 08/03/2010: Western Blot TODO RESULTS
• 09/03/2010: hTERT stimulation @ splitting, Western blot contn'd TODO RESULTS
• 10/03/2010: Start stimulation D9, Stop stimulation D12 TODO RESULTS
• 11/03/2010: Stop stimulation TODO
• 12/03/2010: Splitting & Counting cells TODO
• 15/03/2010: Co-IP & ELISA prep
• 16/03/2010: ELISA & hTERT stimulation
• 17/03/2010: Ht31P toxicity
• 18/03/2010: Statistical analysis
• 22/03/2010: Co-IP & Western blot
• 23/03/2010: Co-IP Part II, hTERT Stimulation & Western blot cont'd
• 24/03/2010: Co-IP Part III, Stimulation stop/Viability staining
• 25/03/2010: ELISA
• 26/03/2010: Last Groninger protocols, cell splitting