User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization

ELISA

1. Viability & ELISA
2. Dose effect curve ELISA
3. ELISA
4. ELISA

Co-IP

1. Co-IP 1 Anouk
2. Co-IP 2 Anouk
3. Co-IP 3 Anouk
4. Co-IP 4 TODO

Misc.

• Ht31P toxicity
• Statistical analysis

• 10/02/2010: Splitting ASM cells
• 12/02/2010: Counting cells
• 15/02/2010: Putting cells to S⁰
• 16/02/2010: Stimulation hTERT cells
• 17/02/2010: Viability staining
• 18/02/2010: ELISA
• 19/02/2010: Counting and splitting cells
• 22/02/2010: Preparing new DMEM S⁺
• 23/02/2010: Putting cells to S⁰, Co-immunoprecipitation
• 24/02/2010: Co-immunoprecipitation part II, (Stimulation hTERT cells v2)
• 25/02/2010: Co-immunoprecipitation part III & Viability staining
• 26/02/2010: ELISA, counting/splitting cells
• 01/03/2010: Western blot
• 03/03/2010: Co-IP day I, GAPDH, VASP, Stop Stimulation HT4
• 04/03/2010: Co-IP day II, ELISA HT4, Cell Stimulation
• 05/03/2010: Co-IP day III, Stop stimulation hTERT, Split hTERT
• 08/03/2010: Western Blot
• 09/03/2010: hTERT stimulation @ splitting, Western blot contn'd
• 10/03/2010: Start stimulation D9, Stop stimulation D12
• 11/03/2010: Stop stimulation
• 12/03/2010: Splitting & Counting cells
• 15/03/2010: Co-IP & ELISA prep
• 16/03/2010: ELISA & hTERT stimulation
• 17/03/2010: Ht31P toxicity
• 18/03/2010: Statistical analysis
• 22/03/2010: Co-IP & Western blot
• 23/03/2010: Co-IP Part II, hTERT Stimulation & Western blot cont'd
• 24/03/2010: Co-IP Part III, Stimulation stop/Viability staining
• 25/03/2010: ELISA
• 26/03/2010: Last Groninger protocols, cell splitting

• 31/03/2010: Building smoke machine, cell splitting
• 01/04/2010: Creating RII overlay protocol
• 06/04/2010: Putting cells to S⁰ and splitting cells
• 07/04/2010: Preparation first Pilot Berlin
• 08/04/2010: ??????????????
• 09/04/2010: Western blot cAMP precipitation for RII overlay
• 11/04/2010: Splitting D9
• 13/04/2010: ??????????????
• 14/04/2010: ??????????????
• 15/04/2010: ??????????????
• ..............
• 07/05/2010: Potter-Elvehjem Cell Homogenizing