User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization

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* 06/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/06|Putting cells to S<sup>0</sup> and splitting cells]]
* 06/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/06|Putting cells to S<sup>0</sup> and splitting cells]]
*07/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/07|Preparation first Pilot Berlin]]
*07/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/07|Preparation first Pilot Berlin]]
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*08/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/08|??????????????]]
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*08/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/08|cAMP precipitation for RII overlay & New cells]]
*09/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/09|Western blot cAMP precipitation for RII overlay]]
*09/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/09|Western blot cAMP precipitation for RII overlay]]
*11/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/11|Splitting D9]]
*11/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/11|Splitting D9]]
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*13/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/13|??????????????]]
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*13/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/13|RII overlay, splitting cells]]
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*14/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/14|??????????????]]
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*14/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/14|RII overlay & Western blot]]
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*15/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/15|??????????????]]
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*15/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/15|SDS-PAGE (MARKERS) and Immunoblot]]
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*16/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/16|Split cells for ELISA and secondary antibody Immunoblot]]
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*18/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/18|Refreshed medium for ELISA cells]]
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*19/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/19|Put cells to S<sup>0</sup> for cAMP precipitation, looked into viability staining (MTT Assay)]]
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*20/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/20|Put cells to S<sup>0</sup> for ELISA, stimulation for cAMP precipitation]]
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*21/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/21|cAMP precipitation, stimulation for ELISA]]
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*22/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/22|Stopping ELISA stimulation, preparing gels]]
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*23/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/23|ELISA]]
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*26/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/26|SDS-PAGE & Refreshed medium]]
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*27/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/27|RII Overlay]]
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*28/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/28|RII Overlay & Split cells]]
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*29/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/29|SDS-PAGE& Finalizing RII overlay]]
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*..............
*..............
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*07/05/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/05/07|Potter-Elvehjem Cell Homogenizing]]
*07/05/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/05/07|Potter-Elvehjem Cell Homogenizing]]
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*..............
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*10/05/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/05/10|Refreshed medium]]
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*11/05/2010:[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/05/11|Split cells]]
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*19/05/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/05/19|RII overlay cAMP precipitation]]
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Revision as of 10:43, 26 May 2010

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Project Description/Abstract

“Towards insights into cyclic AMP compartmentalization in cell of lung origin” a cooperation between the department of Molecular Pharmacology (University Groningen, the Netherlands) and the Leibniz-Institut für Molekulare Pharmakologie (Berlin, Germany). These are the entries of a Master internship from February 'till September 2010.

Notes

Interesting entries

Protocols

Results

ELISA
  1. Viability & ELISA
  2. Dose effect curve ELISA
  3. ELISA
  4. ELISA
Co-IP
  1. Co-IP 1 Anouk
  2. Co-IP 2 Anouk
  3. Co-IP 3 Anouk
  4. Co-IP 4 TODO
Misc.

Convenient Links

Downloads

Blanc for IL-8 ELISA (Linear regression)

Blanc for protein size on gel/membrane (non-Linear regression)

Table of contents

'
Groningen Berlin
  • ..............

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