User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization

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*28/05/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/05/28|Immunoblot AKAP79 (upstate)]]
*28/05/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/05/28|Immunoblot AKAP79 (upstate)]]
*31/05/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/05/31|SDS-PAGE for AKAP79 immunoblot & RII overlay (HEK293)]]
*31/05/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/05/31|SDS-PAGE for AKAP79 immunoblot & RII overlay (HEK293)]]
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*01/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/01|SDS-PAGE for immunoblot (fail)]]
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*02/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/02|Preparing TRIS Urea gel]]
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*03/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/03|Tris urea gel]]
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*07/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/07|Urea-TRIS page, splitting cells]]
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*08/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/08|Creating RNA isolation protocol]]
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*09/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/09|Refreshed medium]]
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*10/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/10|Put cells to S<sup>0</sup>, split cells]]
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*11/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/11|Cell stimulation]]
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Revision as of 04:22, 11 June 2010

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Project Description/Abstract

“Towards insights into cyclic AMP compartmentalization in cell of lung origin” a cooperation between the department of Molecular Pharmacology (University Groningen, the Netherlands) and the Leibniz-Institut für Molekulare Pharmakologie (Berlin, Germany). These are the entries of a Master internship from February 'till September 2010.

Notes

Interesting entries

Protocols

Results

ELISA
  1. Viability & ELISA
  2. Dose effect curve ELISA
  3. ELISA
  4. ELISA
  5. ..
  6. ELISA Berlin Okay
Co-IP
  1. Co-IP 1 Anouk
  2. Co-IP 2 Anouk
  3. Co-IP 3 Anouk
  4. Co-IP 4 TODO
RII Overlay
  1. ..
  2. RII overlay Okay
Misc.

Convenient Links

Downloads

Blanc for IL-8 ELISA (Linear regression)

Blanc for protein size on gel/membrane (non-Linear regression)

Table of contents

'
Groningen Berlin

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