User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization

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*10/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/10|Put cells to S<sup>0</sup>, split cells]]
*10/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/10|Put cells to S<sup>0</sup>, split cells]]
*11/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/11|Cell stimulation]]
*11/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/11|Cell stimulation]]
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*12/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/12|Stop stimulation: Protein and RNA/Protein isolation]]
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*13/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/13|Putting cells to S<sup>0</sup>, splitting cells and continuing of protein isolation]]
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*14/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/14|Cell stimulation]]
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*15/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/15|Stop stimulation: Protein and RNA/Protein isolation (2)]]
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*16/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/16|Stimulation of cells]]
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*17/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/17|Stop stimulation: Protein and RNA/Protein isolation (3)]]
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*21/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/21|New HEK293, Bradford assay, cAMP precipitation]]
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*22/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/22|cAMP precipitation]]
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*23/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/23|Immunoblot]]
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Revision as of 04:08, 23 June 2010

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Project Description/Abstract

“Towards insights into cyclic AMP compartmentalization in cell of lung origin” a cooperation between the department of Molecular Pharmacology (University Groningen, the Netherlands) and the Leibniz-Institut für Molekulare Pharmakologie (Berlin, Germany). These are the entries of a Master internship from February 'till September 2010.

Notes

Interesting entries

Protocols

Results

ELISA
  1. Viability & ELISA
  2. Dose effect curve ELISA
  3. ELISA
  4. ELISA
  5. ..
  6. ELISA Berlin Okay
Co-IP
  1. Co-IP 1 Anouk
  2. Co-IP 2 Anouk
  3. Co-IP 3 Anouk
  4. Co-IP 4 TODO
RII Overlay
  1. ..
  2. RII overlay Okay
Misc.

Convenient Links

Downloads

Blanc for IL-8 ELISA (Linear regression)

Blanc for protein size on gel/membrane (non-Linear regression)

Table of contents

'
Groningen Berlin

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