User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization

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(Protocols)
(Protocols)
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* cAMP-precipitarion [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/08|Part I]] & [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/09#Washing_beads:_cAMP_precipitation|Part II]]
* cAMP-precipitarion [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/08|Part I]] & [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/09#Washing_beads:_cAMP_precipitation|Part II]]
** [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/27#Summary|RIPA Buffer (Berlin)]]
** [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/27#Summary|RIPA Buffer (Berlin)]]
 +
** [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/08#Discussion|RIPA Buffer (Groningen)]]
* Protein concentration determination: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/24|Pierce determination]] & [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/21|Bradford assay]]
* Protein concentration determination: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/24|Pierce determination]] & [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/21|Bradford assay]]
* [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/25#Coomassie_staining|Coomassie staining]]
* [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/25#Coomassie_staining|Coomassie staining]]

Revision as of 09:11, 8 July 2010

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Project Description/Abstract

“Towards insights into cyclic AMP compartmentalization in cell of lung origin” a cooperation between the department of Molecular Pharmacology (University Groningen, the Netherlands) and the Leibniz-Institut für Molekulare Pharmakologie (Berlin, Germany). These are the entries of a Master internship from February 'till September 2010.

Notes

Interesting entries

Protocols

Results

ELISA
  1. Viability & ELISA
  2. Dose effect curve ELISA
  3. ELISA
  4. ELISA
  5. ..
  6. ELISA Berlin Okay
Co-IP
  1. Co-IP 1 Anouk
  2. Co-IP 2 Anouk
  3. Co-IP 3 Anouk
  4. Co-IP 4 TODO
RII Overlay
  1. ..
  2. RII overlay Okay
Misc.

Convenient Links

Downloads

Blanc for IL-8 ELISA (Linear regression)

Blanc for protein size on gel/membrane (non-Linear regression)

Table of contents

'
Groningen Berlin

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