User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization

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<b>February</b>
* 10/02/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/10| Splitting ASM cells]]
* 10/02/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/10| Splitting ASM cells]]
* 12/02/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/12| Counting cells]]
* 12/02/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/12| Counting cells]]
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* 25/02/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/25| Co-immunoprecipitation part III & Viability staining]]  
* 25/02/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/25| Co-immunoprecipitation part III & Viability staining]]  
* 26/02/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/26| ELISA, counting/splitting cells]]
* 26/02/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/26| ELISA, counting/splitting cells]]
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<b>March</b>
* 01/03/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/03/01| Western blot]]
* 01/03/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/03/01| Western blot]]
* 03/03/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/03/03| Co-IP day I, GAPDH, VASP, Stop Stimulation HT4]]  
* 03/03/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/03/03| Co-IP day I, GAPDH, VASP, Stop Stimulation HT4]]  
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* 31/03/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/03/31|Building smoke machine, cell splitting]]
* 31/03/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/03/31|Building smoke machine, cell splitting]]
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<b>April</b>
* 01/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/01|Creating RII overlay protocol]]
* 01/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/01|Creating RII overlay protocol]]
* 06/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/06|Putting cells to S<sup>0</sup> and splitting cells]]
* 06/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/06|Putting cells to S<sup>0</sup> and splitting cells]]
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*29/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/29|SDS-PAGE& Finalizing RII overlay]]
*29/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/29|SDS-PAGE& Finalizing RII overlay]]
*30/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/30|Splitting cells, Immunoblot]]
*30/04/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/30|Splitting cells, Immunoblot]]
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<b>May</b>
*03/05/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/05/03|Split cells for RII overlay]]
*03/05/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/05/03|Split cells for RII overlay]]
*05/05/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/05/05|Put cells to S<sup>0</sup> for RII overlay
*05/05/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/05/05|Put cells to S<sup>0</sup> for RII overlay
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*28/05/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/05/28|Immunoblot AKAP79 (upstate)]]
*28/05/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/05/28|Immunoblot AKAP79 (upstate)]]
*31/05/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/05/31|SDS-PAGE for AKAP79 immunoblot & RII overlay (HEK293)]]
*31/05/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/05/31|SDS-PAGE for AKAP79 immunoblot & RII overlay (HEK293)]]
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<b>June</b>
*01/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/01|SDS-PAGE for immunoblot (fail)]]
*01/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/01|SDS-PAGE for immunoblot (fail)]]
*02/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/02|Preparing TRIS Urea gel]]
*02/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/02|Preparing TRIS Urea gel]]
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*24/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/24|Immunoblot repeat]]
*24/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/24|Immunoblot repeat]]
*25/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/25|Coomassie staining]]
*25/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/25|Coomassie staining]]
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*28/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/28|HeLa lysis, Bradford & cAMP precipitation part I]]
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*29/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/29|cAMP precipitation part II & SDS-PAGE]]
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*30/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/30|Lyse HeLa]]
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*01/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/01|Bradford, qPCR AKAP12 & AKAP9, Silverstaining]]
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*02/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/02|xx]]
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*03/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/03|xx]]
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*05/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/05|xx]]
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*06/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/06|xx]]
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*07/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/07|xx]]
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*08/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/08|xx]]
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*09/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/09|xx]]
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*10/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/10|xx]]
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*11/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/11|xx]]
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*12/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/12|xx]]
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*13/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/13|xx]]
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*14/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/14|xx]]
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*15/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/15|xx]]
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*16/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/16|xx]]
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*19/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/19|xx]]
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*20/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/20|xx]]
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*21/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/21|xx]]
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*24/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/24|xx]]
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*25/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/25|xx]]
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*26/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/26|xx]]
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*27/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/27|xx]]
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*28/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/28|xx]]
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*29/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/29|xx]]
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Revision as of 07:26, 29 July 2010

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Project Description/Abstract

“Towards insights into cyclic AMP compartmentalization in cell of lung origin” a cooperation between the department of Molecular Pharmacology (University Groningen, the Netherlands) and the Leibniz-Institut für Molekulare Pharmakologie (Berlin, Germany). These are the entries of a Master internship from February 'till September 2010.

Notes

Interesting entries

Protocols

Results

ELISA
  1. Viability & ELISA
  2. Dose effect curve ELISA
  3. ELISA
  4. ELISA
  5. ..
  6. ELISA Berlin Okay
Co-IP
  1. Co-IP 1 Anouk
  2. Co-IP 2 Anouk
  3. Co-IP 3 Anouk
  4. Co-IP 4 TODO
RII Overlay
  1. ..
  2. RII overlay Okay
Misc.

Convenient Links

Downloads

Blanc for IL-8 ELISA (Linear regression)

Blanc for protein size on gel/membrane (non-Linear regression)

Table of contents

'
Groningen Berlin

February

March

April

May

June

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