User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization

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*29/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/29|cAMP precipitation part II & SDS-PAGE]]
*29/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/29|cAMP precipitation part II & SDS-PAGE]]
*30/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/30|Lyse HeLa]]
*30/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/30|Lyse HeLa]]
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<b>July</b>
*01/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/01|Bradford, qPCR AKAP12 & AKAP9, Silverstaining]]
*01/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/01|Bradford, qPCR AKAP12 & AKAP9, Silverstaining]]
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*02/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/02|xx]]
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*02/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/02|qPCR GAPDH, Silverstaining]]
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*03/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/03|xx]]
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*03/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/03|Silver staining attempt #3]]
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*05/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/05|xx]]
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*05/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/05|Bradford & cAMP precipitation]]
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*06/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/06|xx]]
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*06/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/06|cAMP precipitation gel]]
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*07/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/07|xx]]
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*07/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/07|RII overlay cAMP precipation]]
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*08/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/08|xx]]
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*08/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/08|RII overlay result]]
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*09/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/09|xx]]
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*09/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/09|Gravin Co-IP, cAMP precipation gel]]
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*10/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/10|xx]]
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*10/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/10|Gravin Co-IP gel]]
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*11/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/11|xx]]
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*11/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/11|AKAP250 (scbt) immunoblot cAMP precipitation]]
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*12/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/12|xx]]
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*12/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/12|AKAP250 (scbt) immunoblot AKAP250 Co-IP]]
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*13/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/13|xx]]
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*{{todo}} 13/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/13|AKAP250 (Eurogentech) immunoblot]]
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*14/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/14|xx]]
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*{{todo}} 14/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/14|AKAP250 (abcam) immunoblot]]
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*15/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/15|xx]]
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*15/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/15|Epac immunoblot]]
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*16/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/16|xx]]
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*{{todo}} 16/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/16|Epac immunoblot result]]
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*19/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/19|xx]]
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*19/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/19|Epac immunoblot repeat, new cell culture]]
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*20/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/20|xx]]
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*{{todo}} 20/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/20|Epac immunoblot repeat result]]
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*21/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/21|xx]]
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*21/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/21|Splitting cells]]
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*24/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/24|xx]]
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*24/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/24|Splitting cells for immunofluorescence]]
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*25/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/25|xx]]
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*25/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/25|Cell check]]
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*26/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/26|xx]]
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*26/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/26|Cover slips to S<sup>0</sup> & Plating out for ELISA]]
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*27/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/27|xx]]
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*27/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/27|Stimulation cover slips, PKA-Co-IP]]
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*28/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/28|xx]]
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*28/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/28|Immunofluorescence for cells, Gravin & RII localization]]
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*29/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/29|xx]]
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*29/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/29|PKA-Co-IP gel]]
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Revision as of 08:50, 29 July 2010

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Project Description/Abstract

“Towards insights into cyclic AMP compartmentalization in cell of lung origin” a cooperation between the department of Molecular Pharmacology (University Groningen, the Netherlands) and the Leibniz-Institut für Molekulare Pharmakologie (Berlin, Germany). These are the entries of a Master internship from February 'till September 2010.

Notes

Interesting entries

Protocols

Results

ELISA
  1. Viability & ELISA
  2. Dose effect curve ELISA
  3. ELISA
  4. ELISA
  5. ..
  6. ELISA Berlin Okay
Co-IP
  1. Co-IP 1 Anouk
  2. Co-IP 2 Anouk
  3. Co-IP 3 Anouk
  4. Co-IP 4 TODO
RII Overlay
  1. ..
  2. RII overlay Okay
Misc.

Convenient Links

Downloads

Blanc for IL-8 ELISA (Linear regression)

Blanc for protein size on gel/membrane (non-Linear regression)

Table of contents

'
Groningen Berlin

February

March

April

May

June

July

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