User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization

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*20/08/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/20|Stripping blots, qPCR AKAP8, refresh medium new cells, 2<sup>nd AB]]
*20/08/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/20|Stripping blots, qPCR AKAP8, refresh medium new cells, 2<sup>nd AB]]
*21/08/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/21|Lysate SDS-PAGE, qPCR AKAP5, Western blot AKAP79 and Gravin]]
*21/08/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/21|Lysate SDS-PAGE, qPCR AKAP5, Western blot AKAP79 and Gravin]]
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*23/08/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/23|SDS-PAGE cAMP precipitation, pre-IgG antibody]]
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Revision as of 13:42, 23 August 2010

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Project Description/Abstract

“Towards insights into cyclic AMP compartmentalization in cell of lung origin” a cooperation between the department of Molecular Pharmacology (University Groningen, the Netherlands) and the Leibniz-Institut für Molekulare Pharmakologie (Berlin, Germany). These are the entries of a Master internship from February 'till September 2010.

Notes

Interesting entries

Protocols

Results

ELISA
  1. Viability & ELISA
  2. Dose effect curve ELISA
  3. ELISA
  4. ELISA
  5. ..
  6. ELISA Berlin Okay
Co-IP
  1. Co-IP 1 Anouk
  2. Co-IP 2 Anouk
  3. Co-IP 3 Anouk
  4. Co-IP 4 TODO
RII Overlay
  1. ..
  2. RII overlay Okay
Misc.

Convenient Links

Downloads

Blanc for IL-8 ELISA (Linear regression)

Blanc for protein size on gel/membrane (non-Linear regression)

Blanc form IF coverslips

Table of contents

'
Groningen Berlin

February

March

April

May

June

July

August


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