User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization

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* Presentation [[Image:PRESENTATION_POWERPOINT_August2010.ppt|August]]
====Convenient Links====
====Convenient Links====
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</html> [[Media:BLANCO_PROTEIN_SIZE.xls| Blanc for protein size on gel/membrane (non-Linear regression)]]
</html> [[Media:BLANCO_PROTEIN_SIZE.xls| Blanc for protein size on gel/membrane (non-Linear regression)]]
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<a href="http://openwetware.org/wiki/Image:BLANCO_IF_COPY_ME_BEFORE_USE.xls.xls">
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  <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">
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</html> [[Media:BLANCO_IF_COPY_ME_BEFORE_USE.xls.xls| Blanc form IF coverslips]]
==Table of contents==
==Table of contents==
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*29/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/29|cAMP precipitation part II & SDS-PAGE]]
*29/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/29|cAMP precipitation part II & SDS-PAGE]]
*30/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/30|Lyse HeLa]]
*30/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/30|Lyse HeLa]]
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<b>July</b>
*01/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/01|Bradford, qPCR AKAP12 & AKAP9, Silverstaining]]
*01/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/01|Bradford, qPCR AKAP12 & AKAP9, Silverstaining]]
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*02/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/02|xx]]
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*02/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/02|qPCR GAPDH, Silverstaining]]
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*03/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/03|xx]]
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*03/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/03|Silver staining attempt #3]]
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*05/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/05|xx]]
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*05/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/05|Bradford & cAMP precipitation]]
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*06/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/06|xx]]
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*06/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/06|cAMP precipitation gel]]
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*07/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/07|xx]]
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*07/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/07|RII overlay cAMP precipation]]
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*08/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/08|xx]]
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*08/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/08|RII overlay result]]
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*09/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/09|xx]]
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*09/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/09|Gravin Co-IP, cAMP precipation gel]]
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*10/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/10|xx]]
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*10/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/10|Gravin Co-IP gel]]
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*11/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/11|xx]]
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*11/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/11|AKAP250 (scbt) immunoblot cAMP precipitation]]
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*12/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/12|xx]]
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*12/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/12|AKAP250 (scbt) immunoblot AKAP250 Co-IP]]
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*13/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/13|xx]]
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*{{todo}} 13/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/13|AKAP250 (Eurogentech) immunoblot]]
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*14/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/14|xx]]
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*{{todo}} 14/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/14|AKAP250 (abcam) immunoblot]]
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*15/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/15|xx]]
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*15/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/15|Epac immunoblot]]
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*16/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/16|xx]]
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*{{todo}} 16/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/16|Epac immunoblot result]]
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*19/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/19|xx]]
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*19/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/19|Epac immunoblot repeat, new cell culture]]
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*20/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/20|xx]]
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*{{todo}} 20/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/20|Epac immunoblot repeat result]]
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*21/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/21|xx]]
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*21/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/21|Splitting cells]]
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*24/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/24|xx]]
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*24/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/24|Splitting cells for immunofluorescence]]
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*25/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/25|xx]]
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*25/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/25|Cell check]]
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*26/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/26|xx]]
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*26/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/26|Cover slips to S<sup>0</sup> & Plating out for ELISA]]
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*27/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/27|xx]]
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*27/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/27|Stimulation cover slips, PKA-Co-IP]]
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*28/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/28|xx]]
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*28/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/28|Immunofluorescence for cells, Gravin & RII localization]]
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*29/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/29|xx]]
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*29/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/29|PKA-Co-IP gel]]
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*30/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/30|Trypsin/EDTA dilution, splitting cells for ELISA]]
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*31/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/31|Put cells to S<sup>0</sup>]]
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<b>August</b>
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*01/08/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/01|Stimulation for ELISA]]
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*02/08/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/02|Put cells to S<sup>0</sup>, Splitting cells, stopping stimulation]]
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*03/08/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/03|ELISA, RII, IF]]
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*04/08/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/04|Stop stimulation, put IF to S<sup>0</sup>]]
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*05/08/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/05|Start stimulation ELISA & IF, splitting cells]]
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*06/08/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/06|Ending stimulation ELISA, Primary antibody IF]]
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*07/08/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/07|Finish IF, Split cells]]
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*09/08/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/09|Isolate Protein/RNA Jurkat cells, Codes IF]]
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*10/08/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/10|ELISA, qPCR Jurkat, SDS-PAGE cAMP precipitation]]
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*11/08/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/11|Jurkat qPCR results, Primary antibody WB, Ending stimulation ELISA]]
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*12/08/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/12|Lyse HEK293 (isolate RNA), 2<sup>nd</sup> AB WB, Convocal microscopy]]
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*13/08/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/13|SDS-PAGE Lysates, strip blot]]
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*14/08/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/14|Secondary antibody cAMP precipitation blot of Gravin en AKAP79]]
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*16/08/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/16|SDS-PAGE lysates, Cell splitting]]
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*17/08/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/17|ON Blot lysate samples]]
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*18/08/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/18|BioRad workshop]]
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*19/08/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/19|Prepare cDNA, Immunoblot visualization, 1<sup>st</sup> AB ON Blot]]
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*20/08/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/20|Stripping blots, qPCR AKAP8, refresh medium new cells, 2<sup>nd AB]]
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*21/08/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/21|Lysate SDS-PAGE, qPCR AKAP5, Western blot AKAP79 and Gravin]]
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*23/08/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/23|SDS-PAGE cAMP precipitation, pre-IgG antibody]]
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*24/08/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/24|RII Overlay, Pre-IgG 2<sup>nd</sup> AB, CSE on HEK293, ASMC to S<sup>0</sup>, Split cells w. Mileh]]
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*25/08/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/25|RII Overlay all]]
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Project Description/Abstract

“Towards insights into cyclic AMP compartmentalization in cell of lung origin” a cooperation between the department of Molecular Pharmacology (University Groningen, the Netherlands) and the Leibniz-Institut für Molekulare Pharmakologie (Berlin, Germany). These are the entries of a Master internship from February 'till September 2010.

Notes

Interesting entries

Protocols

Results

ELISA
  1. Viability & ELISA
  2. Dose effect curve ELISA
  3. ELISA
  4. ELISA
  5. ..
  6. ELISA Berlin Okay
Co-IP
  1. Co-IP 1 Anouk
  2. Co-IP 2 Anouk
  3. Co-IP 3 Anouk
  4. Co-IP 4 TODO
RII Overlay
  1. ..
  2. RII overlay Okay
Misc.

Convenient Links

Downloads

Blanc for IL-8 ELISA (Linear regression)

Blanc for protein size on gel/membrane (non-Linear regression)

Blanc form IF coverslips

Table of contents

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Groningen Berlin

February

March

April

May

June

July

August


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