Project Description/Abstract
“Towards insights into cyclic AMP compartmentalization in cell of lung origin” a cooperation between the department of Molecular Pharmacology (University Groningen, the Netherlands) and the Leibniz-Institut für Molekulare Pharmakologie (Berlin, Germany). These are the entries of a Master internship from February 'till September 2010.
Notes
Interesting entries
Protocols
Results
Convenient Links
Table of contents
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| Groningen
| Berlin
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- 10/02/2010: Splitting ASM cells
- 12/02/2010: Counting cells
- 15/02/2010: Putting cells to S0
- 16/02/2010: Stimulation hTERT cells
- 17/02/2010: Viability staining
- 18/02/2010: ELISA
- 19/02/2010: Counting and splitting cells
- 22/02/2010: Preparing new DMEM S+
- 23/02/2010: Putting cells to S0, Co-immunoprecipitation
- 24/02/2010: Co-immunoprecipitation part II, (Stimulation hTERT cells v2)
- 25/02/2010: Co-immunoprecipitation part III & Viability staining RESULTS
- 26/02/2010: ELISA, counting/splitting cells RESULTS
- 01/03/2010: Western blot RESULTS
- 03/03/2010: Co-IP day I, GAPDH, VASP, Stop Stimulation HT4 TODO
- 04/03/2010: Co-IP day II, ELISA HT4, Cell Stimulation TODO
- 05/03/2010: Co-IP day III, Stop stimulation hTERT, Split hTERT TODO
- 08/03/2010: Western Blot TODO RESULTS
- 09/03/2010: hTERT stimulation @ splitting, Western blot contn'd TODO RESULTS
- 10/03/2010: Start stimulation D9, Stop stimulation D12 TODO RESULTS
- 11/03/2010: Stop stimulation TODO
- 12/03/2010: Splitting & Counting cells TODO
- 15/03/2010: Co-IP & ELISA prep
- 16/03/2010: ELISA & hTERT stimulation
- 17/03/2010: Ht31P toxicity
- 18/03/2010: Statistical analysis
- 22/03/2010: Co-IP & Western blot
- 23/03/2010: Co-IP Part II, hTERT Stimulation & Western blot cont'd
- 24/03/2010: Co-IP Part III, Stimulation stop/Viability staining
- 25/03/2010: ELISA
- 26/03/2010: Last Groninger protocols, cell splitting
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