User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/02/17: Difference between revisions
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!A | !A | ||
|1A1 | |style="background:red; color:white" |1A1 | ||
|1A2 | |style="background:red; color:white" |1A2 | ||
|1A3 | |style="background:red; color:white" |1A3 | ||
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|1A5 | |style="background:red; color:white" |1A5 | ||
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|2A1 | |style="background:blue; color:white" |2A1 | ||
|2A2 | |style="background:blue; color:white" |2A2 | ||
|2A3 | |style="background:blue; color:white" |2A3 | ||
|2A4 | |style="background:blue; color:white" |2A4 | ||
|2A5 | |style="background:blue; color:white" |2A5 | ||
|2A6 | |style="background:blue; color:white" |2A6 | ||
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!B | !B | ||
|1B1 | |style="background:red; color:white" |1B1 | ||
|1B2 | |style="background:red; color:white" |1B2 | ||
|1B3 | |style="background:red; color:white" |1B3 | ||
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|1B6 | |style="background:red; color:white" |1B6 | ||
|2B1 | |style="background:blue; color:white" |2B1 | ||
|2B2 | |style="background:blue; color:white" |2B2 | ||
|2B3 | |style="background:blue; color:white" |2B3 | ||
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|2B5 | |style="background:blue; color:white" |2B5 | ||
|2B6 | |style="background:blue; color:white" |2B6 | ||
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!C | !C | ||
|1C1 | |style="background:red; color:white" |1C1 | ||
|1C2 | |style="background:red; color:white" |1C2 | ||
|1C3 | |style="background:red; color:white" |1C3 | ||
|1C4 | |style="background:red; color:white" |1C4 | ||
|1C5 | |style="background:red; color:white" |1C5 | ||
|1C6 | |style="background:red; color:white" |1C6 | ||
|2C1 | |style="background:blue; color:white" |2C1 | ||
|2C2 | |style="background:blue; color:white" |2C2 | ||
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|2C5 | |style="background:blue; color:white" |2C5 | ||
|2C6 | |style="background:blue; color:white" |2C6 | ||
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!D | !D | ||
|1D1 | |style="background:red; color:white" |1D1 | ||
|1D2 | |style="background:red; color:white" |1D2 | ||
|1D3 | |style="background:red; color:white" |1D3 | ||
|1D4 | |style="background:red; color:white" |1D4 | ||
|1D5 | |style="background:red; color:white" |1D5 | ||
|1D6 | |style="background:red; color:white" |1D6 | ||
|2D1 | |style="background:blue; color:white" |2D1 | ||
|2D2 | |style="background:blue; color:white" |2D2 | ||
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!E | !E | ||
|3A1 | |style="background:orange; color:white" |3A1 | ||
|3A2 | |style="background:orange; color:white" |3A2 | ||
|3A3 | |style="background:orange; color:white" |3A3 | ||
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|3B1 | |style="background:orange; color:white" |3B1 | ||
|3B2 | |style="background:orange; color:white" |3B2 | ||
|3B3 | |style="background:orange; color:white" |3B3 | ||
|3B4 | |style="background:orange; color:white" |3B4 | ||
|3B5 | |style="background:orange; color:white" |3B5 | ||
|3B6 | |style="background:orange; color:white" |3B6 | ||
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!G | !G | ||
|3C1 | |style="background:orange; color:white" |3C1 | ||
|3C2 | |style="background:orange; color:white" |3C2 | ||
|3C3 | |style="background:orange; color:white" |3C3 | ||
|3C4 | |style="background:orange; color:white" |3C4 | ||
|3C5 | |style="background:orange; color:white" |3C5 | ||
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!H | !H | ||
|3D1 | |style="background:orange; color:white" |3D1 | ||
|3D2 | |style="background:orange; color:white" |3D2 | ||
|3D3 | |style="background:orange; color:white" |3D3 | ||
|3D4 | |style="background:orange; color:white" |3D4 | ||
|3D5 | |style="background:orange; color:white" |3D5 | ||
|3D6 | |style="background:orange; color:white" |3D6 | ||
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==Results== | ==Results== | ||
===Alamar Blue=== | |||
{| border="1" | {| border="1" | ||
|+'''D9 (passage 21)''' | |+'''D9 (passage 21)''' | ||
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|- | |- | ||
!A (CTR) | !A (CTR) | ||
|1824 | |style="background:red" |1824 | ||
|1982 | |style="background:red" |1982 | ||
|1953 | |style="background:red" |1953 | ||
|5125 | |style="background:green" |5125 | ||
|5172 | |style="background:green" |5172 | ||
|5939 | |style="background:green" |5939 | ||
|- | |- | ||
!B (CSE) | !B (CSE) | ||
|956 | |style="background:red" |956 | ||
|964 | |style="background:red" |964 | ||
|785 | |style="background:red" |785 | ||
|4465 | |style="background:green" |4465 | ||
|5006 | |style="background:green" |5006 | ||
|5815 | |style="background:green" |5815 | ||
|- | |- | ||
!C (cse +8p) | !C (cse +8p) | ||
|1075 | |style="background:red" |1075 | ||
|1104 | |style="background:red" |1104 | ||
|971 | |style="background:red" |971 | ||
|5017 | |style="background:green" |5017 | ||
|5241 | |style="background:green" |5241 | ||
|5526 | |style="background:green" |5526 | ||
|- | |- | ||
!D (cse+6Bnz) | !D (cse+6Bnz) | ||
|761 | |style="background:red" |761 | ||
|674 | |style="background:red" |674 | ||
|645 | |style="background:red" |645 | ||
|4408 | |style="background:green" |4408 | ||
|5385 | |style="background:green" |5385 | ||
|4943 | |style="background:green" |4943 | ||
|- | |- | ||
|} | |} | ||
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|- | |- | ||
!A (CTR) | !A (CTR) | ||
|2232 | |style="background:red" |2232 | ||
|2560 | |style="background:red" |2560 | ||
|2506 | |style="background:red" |2506 | ||
|6431 | |style="background:green" |6431 | ||
|6394 | |style="background:green" |6394 | ||
|7264 | |style="background:green" |7264 | ||
|- | |- | ||
!B (CSE) | !B (CSE) | ||
|1113 | |style="background:red" |1113 | ||
|1050 | |style="background:red" |1050 | ||
|998 | |style="background:red" |998 | ||
|6077 | |style="background:green" |6077 | ||
|6513 | |style="background:green" |6513 | ||
|7171 | |style="background:green" |7171 | ||
|- | |- | ||
!C (cse +8p) | !C (cse +8p) | ||
|1224 | |style="background:red" |1224 | ||
|1024 | |style="background:red" |1024 | ||
|1234 | |style="background:red" |1234 | ||
|5606 | |style="background:green" |5606 | ||
|6203 | |style="background:green" |6203 | ||
|7559 | |style="background:green" |7559 | ||
|- | |- | ||
!D (cse+6Bnz) | !D (cse+6Bnz) | ||
|730 | |style="background:red" |730 | ||
|826 | |style="background:red" |826 | ||
|858 | |style="background:red" |858 | ||
|5309 | |style="background:green" |5309 | ||
|4856 | |style="background:green" |4856 | ||
|2518 | |style="background:green" |2518 | ||
|- | |- | ||
|} | |} | ||
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|- | |- | ||
!A (CTR) | !A (CTR) | ||
|2359 | |style="background:red" |2359 | ||
|2494 | |style="background:red" |2494 | ||
|2434 | |style="background:red" |2434 | ||
|5865 | |style="background:green" |5865 | ||
|6713 | |style="background:green" |6713 | ||
|6843 | |style="background:green" |6843 | ||
|- | |- | ||
!B (CSE) | !B (CSE) | ||
|998 | |style="background:red" |998 | ||
|929 | |style="background:red" |929 | ||
|957 | |style="background:red" |957 | ||
|5129 | |style="background:green" |5129 | ||
|5632 | |style="background:green" |5632 | ||
|6518 | |style="background:green" |6518 | ||
|- | |- | ||
!C (cse +8p) | !C (cse +8p) | ||
|946 | |style="background:red" |946 | ||
|1033 | |style="background:red" |1033 | ||
|1026 | |style="background:red" |1026 | ||
|5562 | |style="background:green" |5562 | ||
|5622 | |style="background:green" |5622 | ||
|6125 | |style="background:green" |6125 | ||
|- | |- | ||
!D (cse+6Bnz) | !D (cse+6Bnz) | ||
|940 | |style="background:red" |940 | ||
|1123 | |style="background:red" |1123 | ||
|982 | |style="background:red" |982 | ||
|6428 | |style="background:green" |6428 | ||
|5590 | |style="background:green" |5590 | ||
|4116 | |style="background:green" |4116 | ||
|- | |- | ||
|} | |} | ||
Line 308: | Line 309: | ||
|- | |- | ||
!A (CTR) | !A (CTR) | ||
|2151 | |style="background:red" |2151 | ||
|2049 | |style="background:red" |2049 | ||
|2021 | |style="background:red" |2021 | ||
|7501 | |style="background:green" |7501 | ||
|7411 | |style="background:green" |7411 | ||
|7507 | |style="background:green" |7507 | ||
|- | |- | ||
!B (CSE) | !B (CSE) | ||
|773 | |style="background:red" |773 | ||
|848 | |style="background:red" |848 | ||
|814 | |style="background:red" |814 | ||
|5447 | |style="background:green" |5447 | ||
|6630 | |style="background:green" |6630 | ||
|6700 | |style="background:green" |6700 | ||
|- | |- | ||
!C (cse +8p) | !C (cse +8p) | ||
|718 | |style="background:red" |718 | ||
|769 | |style="background:red" |769 | ||
|769 | |style="background:red" |769 | ||
|4324 | |style="background:green" |4324 | ||
|4835 | |style="background:green" |4835 | ||
|6349 | |style="background:green" |6349 | ||
|- | |- | ||
!D (cse+6Bnz) | !D (cse+6Bnz) | ||
|877 | |style="background:red" |877 | ||
|874 | |style="background:red" |874 | ||
|1002 | |style="background:red" |1002 | ||
|5387 | |style="background:green" |5387 | ||
|7649 | |style="background:green" |7649 | ||
|6039 | |style="background:green" |6039 | ||
|- | |- | ||
|} | |} | ||
Line 352: | Line 353: | ||
|- | |- | ||
!A (CTR) | !A (CTR) | ||
|1389 | |style="background:red" |1389 | ||
|2124 | |style="background:red" |2124 | ||
|1980 | |style="background:red" |1980 | ||
|7432 | |style="background:green" |7432 | ||
|7069 | |style="background:green" |7069 | ||
|7977 | |style="background:green" |7977 | ||
|- | |- | ||
!B (CSE) | !B (CSE) | ||
|925 | |style="background:red" |925 | ||
|1021 | |style="background:red" |1021 | ||
|972 | |style="background:red" |972 | ||
|4957 | |style="background:green" |4957 | ||
|5760 | |style="background:green" |5760 | ||
|6746 | |style="background:green" |6746 | ||
|- | |- | ||
!C (cse +8p) | !C (cse +8p) | ||
|960 | |style="background:red" |960 | ||
|1030 | |style="background:red" |1030 | ||
|1069 | |style="background:red" |1069 | ||
|5381 | |style="background:green" |5381 | ||
|4675 | |style="background:green" |4675 | ||
|6522 | |style="background:green" |6522 | ||
|- | |- | ||
!D (cse+6Bnz) | !D (cse+6Bnz) | ||
|767 | |style="background:red" |767 | ||
|877 | |style="background:red" |877 | ||
|1138 | |style="background:red" |1138 | ||
|5216 | |style="background:green" |5216 | ||
|6143 | |style="background:green" |6143 | ||
|6754 | |style="background:green" |6754 | ||
|- | |- | ||
|} | |} | ||
Line 396: | Line 397: | ||
|- | |- | ||
!A (CTR) | !A (CTR) | ||
|2071 | |style="background:red" |2071 | ||
|2158 | |style="background:red" |2158 | ||
|2331 | |style="background:red" |2331 | ||
|7252 | |style="background:green" |7252 | ||
|7248 | |style="background:green" |7248 | ||
|7881 | |style="background:green" |7881 | ||
|- | |- | ||
!B (CSE) | !B (CSE) | ||
|1098 | |style="background:red" |1098 | ||
|953 | |style="background:red" |953 | ||
|1087 | |style="background:red" |1087 | ||
|5078 | |style="background:green" |5078 | ||
|5458 | |style="background:green" |5458 | ||
|5958 | |style="background:green" |5958 | ||
|- | |- | ||
!C (cse +8p) | !C (cse +8p) | ||
|926 | |style="background:red" |926 | ||
|1324 | |style="background:red" |1324 | ||
|1106 | |style="background:red" |1106 | ||
|4599 | |style="background:green" |4599 | ||
|4899 | |style="background:green" |4899 | ||
|5503 | |style="background:green" |5503 | ||
|- | |- | ||
!D (cse+6Bnz) | !D (cse+6Bnz) | ||
|908 | |style="background:red" |908 | ||
|738 | |style="background:red" |738 | ||
|957 | |style="background:red" |957 | ||
|4963 | |style="background:green" |4963 | ||
|4311 | |style="background:green" |4311 | ||
|6101 | |style="background:green" |6101 | ||
|- | |- | ||
|} | |} | ||
Line 433: | Line 434: | ||
Samples should have been been into the wells at random to prevent the cells to concentrate in the first samples. | Samples should have been been into the wells at random to prevent the cells to concentrate in the first samples. | ||
==Related== | ===Related entries=== | ||
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/10| Splitting cells]] | ====Run 1: Ht31/Ht31P D9/D12==== | ||
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/12| Counting cells]] | *[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/10|Splitting cells 10Feb2010]] | ||
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/15| Putting | *[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/12|Counting cells 12Feb2010]] | ||
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/16| | *[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/15|Putting to S<sup>0</sup> 15Feb2010]] | ||
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/16|Stimulating cells 16Feb2010]] | |||
==Related topics== | ==Related topics== |
Revision as of 14:17, 18 March 2010
<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>
Viability staining | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Summary
Materials & MethodsMaterialsMethodPreparations ELISA
Alamar Blue staining
ResultsAlamar Blue
ConclusionSamples should have been been into the wells at random to prevent the cells to concentrate in the first samples. Related entriesRun 1: Ht31/Ht31P D9/D12
Related topics20.109(S08):Testing_cell_viability_(Day3)
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