User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/02/18: Difference between revisions
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==Summary== | ==Summary== | ||
To determine the amount of interleukin 8 (IL-8) produced by the airway smooth muscle cells (ASMC's) [http://en.wikipedia.org/wiki/ELISA ELISA] is performed using the PeliKine Compact™ human IL-8 ELISA kit. In this way the effect of PKA, EPAC and CSE on the inflammatory cytokine can be determined. | To determine the amount of interleukin 8 (IL-8) produced by the airway smooth muscle cells (ASMC's) [http://en.wikipedia.org/wiki/ELISA ELISA] is performed using the PeliKine Compact™ human IL-8 ELISA kit. In this way the effect of PKA, EPAC and CSE on the inflammatory cytokine can be determined. | ||
==Materials & Methods== | ==Materials & Methods== | ||
===Materials=== | ===Materials=== | ||
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** 30 mg [http://en.wikipedia.org/wiki/3,3%E2%80%99,5,5%E2%80%99-Tetramethylbenzidine TMB] | ** 30 mg [http://en.wikipedia.org/wiki/3,3%E2%80%99,5,5%E2%80%99-Tetramethylbenzidine TMB] | ||
** 5 mL [http://en.wikipedia.org/wiki/Dimethyl_sulfoxide DMSO] | ** 5 mL [http://en.wikipedia.org/wiki/Dimethyl_sulfoxide DMSO] | ||
===Method=== | ===Method=== | ||
<b><u>Coating of plate</u></b> | <b><u>Coating of plate</u></b> |
Revision as of 08:41, 18 February 2010
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SummaryTo determine the amount of interleukin 8 (IL-8) produced by the airway smooth muscle cells (ASMC's) ELISA is performed using the PeliKine Compact™ human IL-8 ELISA kit. In this way the effect of PKA, EPAC and CSE on the inflammatory cytokine can be determined. Materials & MethodsMaterials
MethodCoating of plate
Preparing Sandwich
Notes
Results
Conclusion
Related
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