User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/02/23: Difference between revisions

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==Conclusion==
==Conclusion==
*{{done}} Ow..
*{{done}} Ow..
==Related==
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/19|  Counting and splitting cells]]
===Same actions===
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/15| Putting cells to S<sup>0</sup>]]


==Related topics==
==Related topics==

Revision as of 05:30, 23 February 2010

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

Putting cells to S0, Co-immunoprecipitation <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

Cells were put to S0 as was supposed to be done yesterday. Co-immunoprecipitation was performed

Materials & Methods

Materials

  • DMEM S0
  • DMEM S+
  • Cells prepared 19Feb2010
  • PBS
  • HT4 cells (neuroblastoma cell line)

Method

Putting cells to S0

  • Cells were put to S0 as described 15Feb2010
    • Cells were washed three times with PBS

Splitting HT4 cells

  • Split HT4 cells as described for hTERT cells
    • Cells of three flasks were resuspended in total volume of 18 mL (3 times 1 mL Trypsin + 15 mL DMEM S+)
    • 3 mL of suspension was diluted in 42 mL of DMEM S+
    • Cells were plated out 15 mL per flask

Co-immunoprecipitation

6-wells
1 2 3
A
B
24-wells
1 2 3 4 5 6
A
B
C
D
96-wells plate
01 02 03 04 05 06 07 08 09 10 11 12
A
B
C
D
E
F
G
H
I

Results

  • WHAT?

Conclusion

  • DONE Ow..

Related

Same actions

Related topics