User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/02/23: Difference between revisions
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====Co-immunoprecipitation part I==== | ====Co-immunoprecipitation part I==== | ||
* Beads were coated with anti-PKA antibodies. | * Beads were coated with anti-PKA antibodies. | ||
** | * For <b>4</b> samples | ||
** 480 μL beads | |||
** Spin down and remove EtOH | ** Spin down and remove EtOH | ||
** Wash with PBS | ** Wash with excess (400 μL) PBS | ||
** Spin down | ** Spin down and remove PBS | ||
** | ** Add 400 μL PBS | ||
** | ** Add 1.8 μL antibody (or not for control) | ||
** Incubate with antibody ON @ 4 °C | ** Incubate with antibody ON @ 4 °C | ||
===Related entries=== | |||
== | ====Run 2: Ht31 Dose/Effect Curve & S<sup>0</sup> D9/D12==== | ||
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/19|Counting cells 19Feb2010]] | |||
== | |||
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*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/19| | |||
===Same actions=== | ===Same actions=== | ||
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/15| Putting cells to S<sup>0</sup>]] | *[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/15| Putting cells to S<sup>0</sup>]] |
Revision as of 14:23, 18 March 2010
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Putting cells to S0, Co-immunoprecipitation | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
SummaryCells were put to S0 as was supposed to be done yesterday. Beads for Co-immunoprecipitation were prepared. Materials & MethodsMaterialsMethodPutting cells to S0
Splitting HT4 cells
Co-immunoprecipitation part I
Related entriesRun 2: Ht31 Dose/Effect Curve & S0 D9/D12Same actionsRelated topics
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