User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/02/24: Difference between revisions
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** 1.06 mg β-glycerolphosphate | ** 1.06 mg β-glycerolphosphate | ||
** 1 mL [http://wikilaboratory.com/index.php?title=RIPA_Buffer RIPA buffer] | ** 1 mL [http://wikilaboratory.com/index.php?title=RIPA_Buffer RIPA buffer] | ||
** 1 μL Apoprotein | ** 1 μL Apoprotein (1 mg/mL) | ||
** 1 μL Leupeptin | ** 1 μL Leupeptin (1 mg/mL) | ||
** 1 μL Pepstatin (A) | ** 1 μL Pepstatin (A) (1 mg/mL) | ||
** 5 μL Na<sub>3</sub>VO<sub>4</sub> | ** 5 μL Na<sub>3</sub>VO<sub>4</sub> | ||
** 5 μL NaF | ** 5 μL NaF (200 mM) | ||
* [[PBS]] | * [[PBS]] | ||
*[http://www.piercenet.com/Products/Browse.cfm?fldID=02020105 Coomassie (Bradford) Protein Assay Kit] | *[http://www.piercenet.com/Products/Browse.cfm?fldID=02020105 Coomassie (Bradford) Protein Assay Kit] |
Revision as of 05:23, 15 March 2010
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Co-immunoprecipitation part II, (Stimulation hTERT cells v2) | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||
SummaryConditions
With a dose response range of 0 - 50 μg/mLug Ht31, instead of HT31P S0 was used. Materials & MethodsMaterials
MethodDetermination of protein concentration
Co-immunoprecipitation
(possible also beads (30 μL) with only sample (200 μL))
Stimulation hTERT (BSc students
Results
RelatedSame actionsRelated topics |