User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/02/24: Difference between revisions
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# Beads w. antibody (30 μL) & sample (200 μL) | # Beads w. antibody (30 μL) & sample (200 μL) | ||
(possible also beads (30 μL) with only sample (200 μL)) | (possible also beads (30 μL) with only sample (200 μL)) | ||
* Incubate ON @ °C | * Incubate ON @ 4 °C | ||
===Stimulation hTERT (BSc students=== | ===Stimulation hTERT (BSc students=== | ||
* HT31P dose response curve (0 - 50 μM) | * HT31P dose response curve (0 - 50 μM) | ||
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==Related== | ===Related entries=== | ||
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/19| | ====Run 2: Ht31 Dose/Effect Curve & S<sup>0</sup> D9/D12==== | ||
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/23| Putting | *[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/19|Counting cells 19Feb2010]] | ||
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/23|Putting to S<sup>0</sup> 23Feb2010]] | |||
===Same actions=== | ===Same actions=== | ||
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/16| Stimulation hTERT cells]] | *[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/16| Stimulation hTERT cells]] | ||
==Related topics== | ==Related topics== | ||
* [[Cell_and_tissue_lysis_hub]] | * [[Cell_and_tissue_lysis_hub]] |
Revision as of 08:02, 23 March 2010
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Co-immunoprecipitation part II, (Stimulation hTERT cells v2) | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||
SummaryConditions
With a dose response range of 0 - 50 μg/mLug Ht31, instead of HT31P S0 was used. Materials & MethodsMaterials
MethodDetermination of protein concentration
Co-immunoprecipitation
(possible also beads (30 μL) with only sample (200 μL))
Stimulation hTERT (BSc students
Results
Related entriesRun 2: Ht31 Dose/Effect Curve & S0 D9/D12Same actionsRelated topics |