User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/02/25: Difference between revisions
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* Centrifuge, 5 min. 14.000 RPM | * Centrifuge, 5 min. 14.000 RPM | ||
* Remove supernatant<sup>*</sup> | * Remove supernatant<sup>*</sup> | ||
* Add 75 μL Laemli buffer | * Add 75 μL Laemli buffer to beads | ||
* Store samples @ -20 °C | * Store samples @ -20 °C | ||
** Alternatively go directly on to Western blot (Boil samples and spin beads down, bring supernatant onto gel) | ** Alternatively go directly on to Western blot (Boil samples and spin beads down, bring supernatant onto gel) | ||
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<sup>*</sup> Be careful not to touch beads | <sup>*</sup> Be careful not to touch beads | ||
====[[wikipedia: VASP_(gene)| VASP]]==== | ====[[wikipedia: VASP_(gene)| VASP]]==== | ||
* Use cells grown by Bachelor students {{todo}} | * Use cells grown by Bachelor students {{todo}} |
Revision as of 03:02, 12 March 2010
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Co-immunoprecipitation part III | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||
SummaryThe samples have been incubated with the (PKA) antibody coated beads and today the beads are washed and stored in Laemli buffer Materials & MethodsMaterials
MethodCo-immunoprecipitation
VASP
Coating of plate
BSc.
RelatedSame actionRelated topics
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