User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/02/26: Difference between revisions

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** S<sup>+</sup> 12.62 mL + 2.38 mL cells (25.000 cells per well 24-wells plate (500 μL)
** S<sup>+</sup> 12.62 mL + 2.38 mL cells (25.000 cells per well 24-wells plate (500 μL)
* D9
* D9
* ELISA {{todo|SEE DOSE EFFECT CURVE}}


==Conclusion==
==Conclusion==

Revision as of 03:42, 12 March 2010

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

ELISA & Counting splitting cells <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Summary

  • Short summary

Materials & Methods

Materials

Method

ELISA

Sample dilution ELISA (Dilution buffer (μL)/Sample (μL))
# HT31 HT31 HT31 HT31P HT31P HT31P
A 100/100 100/100 100/100 100/100 100/100 100/100
B 175/25 175/25 175/25 150/50 150/50 150/50
C 175/25 175/25 175/25 150/50 150/50 150/50
D 175/25 175/25 175/25 150/50 150/50 150/50

Counting and splitting cells

Results

  • HT4 cells
    • 286
    • 249
    • 203
    • 193
  • D12 hTERT cells (15.75×104 cells/mL)
    • 20
    • 12
    • 15
    • 16
    • S+ 12.62 mL + 2.38 mL cells (25.000 cells per well 24-wells plate (500 μL)
  • D9
  • ELISA SEE DOSE EFFECT CURVE

Conclusion

  • DONE Ow..

Related

Same action




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