User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/02/26

From OpenWetWare

< User:Wilfred J. Poppinga | Notebook | cAMP compartmentalization | 2010 | 02
Revision as of 04:23, 23 March 2010 by Wilfred J. Poppinga (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search

ELISA & Counting splitting cells Main project page
Previous entry      Next entry

Summary

  • Short summary

Materials & Methods

Materials

Method

ELISA

Sample dilution ELISA (Dilution buffer (μL)/Sample (μL))
1 2 3 4 5 6
A 100/100 100/100 100/100 100/100 100/100 100/100
B 150/50 150/50 150/50 175/25 175/25 175/25
C 150/50 150/50 150/50 175/25 175/25 175/25
D 150/50 150/50 150/50 175/25 175/25 175/25

Counting and splitting cells

Results

  • HT4 cells
    • 286
    • 249
    • 203
    • 193
  • D12 hTERT cells (15.75×104 cells/mL)
    • 20
    • 12
    • 15
    • 16
    • S+ 12.62 mL + 2.38 mL cells (25.000 cells per well 24-wells plate (500 μL)
  • D12 P28 of Mark hTERT cells (75.5×104 cells/mL)
    • 92
    • 67
    • 83
    • 60
    • 0.43 mL cells in 23 mL for 24-wells, 3.97 mL in 15 mL for 6 wells
  • D9 (53.5×104 cells/mL)
    • 52
    • 55
    • 64
    • 58
    • 0.61 mL in 13 mL and 5.35 mL in 15 mL
  • ELISA SEE DOSE EFFECT CURVE

Conclusion

  • DONE Ow..

Related entries

Run 2: Ht31 Dose/Effect Curve & S0 D9/D12

Same action

Attachment

Excel Dose Effect curve D9
Excel Dose Effect curve D12



Personal tools