User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/03/04: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
mNo edit summary
Line 5: Line 5:
{|{{table}} width="800"
{|{{table}} width="800"
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Co-IP day II, ELISA HT4, Cell Stimulation</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
|-
Line 24: Line 24:
** used kit for human IL-8, HT4 are mouse cells
** used kit for human IL-8, HT4 are mouse cells
====Co-IP day 2====
====Co-IP day 2====
{| border="1"
|+'''6-wells'''
!#
!<u>1</u>
!<u>2</u>
!<u>3</u>
|-
!A
|
|
|
|-
!B
|
|
|
|-
|}
{| border="1"
|+'''24-wells'''
!#
!<u>1</u>
!<u>2</u>
!<u>3</u>
!<u>4</u>
!<u>5</u>
!<u>6</u>
|-
!A
|
|
|
|
|
|
|-
!B
|
|
|
|
|
|
|-
!C
|
|
|
|
|
|
|-
!D
|
|
|
|
|
|
|-
|}
{| border="1"
|+'''96-wells plate'''
|#
!01
!02
!03
!04
!05
!06
!07
!08
!09
!10
!11
!12
|-
!A
|
|
|
|
|
|
|
|
|
|
|
|
|-
!B
|
|
|
|
|
|
|
|
|
|
|
|
|-
!C
|
|
|
|
|
|
|
|
|
|
|
|
|-
!D
|
|
|
|
|
|
|
|
|
|
|
|
|-
!E
|
|
|
|
|
|
|
|
|
|
|
|
|-
!F
|
|
|
|
|
|
|
|
|
|
|
|
|-
!G
|
|
|
|
|
|
|
|
|
|
|
|
|-
!H
|
|
|
|
|
|
|
|
|
|
|
|
|-
!I
|
|
|
|
|
|
|
|
|
|
|
|
|-
|}


==Notes==
==Notes==

Revision as of 02:47, 23 March 2010

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

Co-IP day II, ELISA HT4, Cell Stimulation <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Summary

  • Short summary

Materials & Methods

Materials

Method

Stimulation of cells

  • Instead of HT31P S0 is used

ELISA

  • Performed by BSc. students
    • used kit for human IL-8, HT4 are mouse cells

Co-IP day 2

Notes

  • Some S0 fell into well B5 for D9 during cell stimulation

Results

  • WHAT?
  • Pierce Co-IP 4-3-10
  • Results HT4 Failure due to wrong kit 040310

Conclusion

  • DONE Ow..



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/03/04&oldid=401345"