User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/03/04

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Summary

  • Short summary

Materials & Methods

Materials

Method

Stimulation of cells

  • Instead of HT31P S0 is used

ELISA

  • Performed by BSc. students
    • used kit for human IL-8, HT4 are mouse cells

Co-IP day 2

6-wells
# 1 2 3
A
B
24-wells
# 1 2 3 4 5 6
A
B
C
D
96-wells plate
# 01 02 03 04 05 06 07 08 09 10 11 12
A
B
C
D
E
F
G
H
I

Notes

  • Some S0 fell into well B5 for D9 during cell stimulation

Results

  • WHAT?
  • Pierce Co-IP 4-3-10
  • Results HT4 Failure due to wrong kit 040310

Conclusion

  • DONE Ow..



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