User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/03/05: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Co-IP day III, Stop stimulation hTERT, Split hTERT</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Results==
==Results==
*{{todo| WHAT?}}
* [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/03/16#Attachment| Viability staining]]
* Alamar Blue hTERT 050310 D9/D12 WJP
 
==Conclusion==
*{{done}} Ow..
 


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Revision as of 02:49, 23 March 2010

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

Co-IP day III, Stop stimulation hTERT, Split hTERT <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Summary

  • Short summary

Materials & Methods

Materials

Method

  • Co-IP day III
  • Stop stimulation D9/D12 24-wells induced yesterday (after 24 hours)
    • Alamar Blue
    • .. D9 ELISA samples got lost, D12 was put to 96-wells in freezer
  • Split cells D9/D12
    • 30.000 cells per well for 24-wells
    • 200.000 cells per well for 6 wells
    • 1 million per plate for 10cm petridish

Results


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